Field-Deployable Lab-on-a-Chip Nanosensing Platforms for Health and Environmental Monitoring

摘要：项目2 拟议的项目描述了一个全面的研究计划，以开发和应用自动化领域， 可部署的芯片实验室纳米传感平台，具有高通量、灵敏度和效率，可用于多个领域， 超级基金中与健康和环境监测有关的有害物质的功能分析 研究中心（SRC）。由于广泛的人类活动在全世界造成越来越多的环境挑战， 现代环境监测和分析技术对保护人类健康变得尤为重要。 这将有助于防止有害的工业污染物暴露，并大大提高公众的认识。然而，在这方面， 传统的基于实验室的分析仪器通常是昂贵和笨重的， 由专职人员执行操作程序。这项工作的目标是提供实地- 可部署的多用途芯片实验室技术由新兴的微流体和纳米传感技术实现 用于环境监测和人类健康的技术。具体来说，两个创新 待研究的技术平台是：1）用于高分辨率的微流体打印分析（MPA）系统， 通量高灵敏度生物分子分析（Aim I），以及2）可现场部署的芯片上ELISA平台， 结合定量纳米传感分子测定（目标II-IV），可以从其中 定制并与用于健康和环境监测的移动的设备连接。在开发时， 预计这些技术将有助于多路复用，定量，自动化处理和分析， 人体生物标本和环境样品，灵敏度高，周转快，成本低。初始 研究将涉及测试这些设备，以检测农药及其降解产物， 对我们社区合作伙伴的高度关注。更广泛地说，这些平台将很容易适应商业 生物识别分子和众多超级基金优先化学品的检测。总的来说，这项研究 该项目直接推进了NIEHS的任务，即开发用于检测危险物质的分析工具。 化学品，并将其应用于环境和人类健康监测应用。

ABSTRACT: PROJECT 2 The proposed project describes a comprehensive research plan to develop and apply automated field- deployable lab-on-a-chip nanosensing platforms with high throughput, sensitivity and efficiency for multi- functional analysis of hazardous substances relevant to health and environmental monitoring in the Superfund Research Center (SRC). As extensive human activities pose increased environmental challenges worldwide, modern environmental monitoring and analytical technologies become particularly important to protect human health from adverse exposure to industrial pollutants, and to considerably improve public awareness. However, conventional laboratory-based analytical instruments are typically expensive and bulky, and require elaborate operational procedures conducted by dedicated personnel. The objective of this work is to deliver field- deployable multipurpose lab-on-a-chip technologies enabled by emerging microfluidics and nanosensing technologies for application in environmental monitoring and human health. Specifically, two innovative technological platforms to be investigated are: 1) a microfluidic print-to-analyze (MPA) system for high- throughput high-sensitivity biomolecular analysis (Aim I), and 2) a field-deployable ELISA-on-a-chip platform to incorporate quantitative nanosensing molecular assays (Aims II-IV), from which a 3D printable device can be customized and interfaced with mobile devices for health and environmental monitoring. Upon development, these technologies are expected to facilitate multiplexed, quantitative, automated processing and analysis of human biospecimens and environmental samples, with high sensitivity, quick turnover at low cost. Initial studies will involve testing these devices to detect pesticides and their degradation products, as an identified high concern for our community partner. More broadly, these platforms will be easily adaptable to commercial biorecognition molecules and detection of numerous Superfund priority chemicals. Overall, this research project directly advances the NIEHS mandate to develop analytical tools for the detection of hazardous chemicals and to apply them to environmental and human health monitoring applications.