Infant Bronchiolitis and Viral Core (IBVC)

项目摘要/摘要 呼吸道合胞病毒(RSV)在儿童中引起一系列疾病，包括上呼吸道 感染严重的下呼吸道疾病，如毛细支气管炎和肺炎。尽管 疾病从上呼吸道到下呼吸道进展的决定因素，毛细支气管炎的严重程度， 复发和长期发病率，如反复喘息或哮喘，还没有完全了解，两者都 宿主和病毒特有的因素似乎对这种感染的发病机制和/或结果起到了作用。 我们P01的长期目标是了解粘膜在病毒和过敏原诱导的呼吸道中所起的作用 炎症和重塑。我们将通过提供新的机制来推进我们的目标和领域 先天病毒和过敏原刺激会导致表观遗传重塑、氧化损伤和细胞重新编程 天然黏膜干扰素(干扰素)反应。婴儿毛细支气管炎和病毒核心(IBVC)的目的是 从一群感染了肺炎的幼儿获取高质量的、经过临床注释的粘膜样本。 呼吸道合胞病毒(RSV)，并提供集中的科学资源，支持对 优化、同质(高度重复性)的RSV制剂、免疫分析性能和使用 最新的实验小鼠感染模型，这是研究项目(RP)RP1-RP3所需的 我们的P01。样本和数据的有效管理和分发将确保项目的成功。 此外，IBVC还将为P01中心提供服务、技术和智力援助 功能齐全、经济高效的多用户环境中的调查人员，这将提高双方的工作效率 个人和合作研究计划。除了最先进的设备和方法，关键是 IBVC的人员提供科学和技术专业知识，使中心的调查人员在 实验设计、执行和解释方面的条款，以及通过提供协作的重点 互动。

PROJECT SUMMARY/ABSTRACT Respiratory syncytial virus (RSV) causes a spectrum of disease in children ranging from upper respiratory infections to severe lower respiratory tract disease, such as bronchiolitis and pneumonia. Although the determinants of disease progression from the upper to the lower respiratory tract, severity of bronchiolitis, recurrences, and long-term morbidity, such as recurrent wheezing or asthma, are not fully understood, both host- and virus-specific factors appear to contribute to the pathogenesis and/or the outcome of this infection. The long-term goal of our P01 is to understand the role the mucosa plays in virus- and allergen-induced airway inflammation and remodeling. We will advance our aims and the field by providing new mechanisms by which innate viral and allergen stimuli produce epigenetic remodeling, oxidative injury and reprogramming of the innate mucosal interferon (IFN) response. The purpose of the Infant Bronchiolitis and Viral Core (IBVC) is to acquire high-quality, clinically annotated mucosal samples from a cohort of young children infected with respiratory syncytial virus (RSV), and to provide a centralized scientific resource that supports the need for optimized, homogeneous (highly reproducible) RSV preparations, immunoassay performance, and use of state-of-the art experimental mouse models of infection, which are required in research project (RP) RP1-RP3 of our P01. Efficient management and distribution of samples and data will ensure the success of the projects. Furthermore, the IBVC will provide services, technical and intellectual assistance to the P01 Center investigators in a functional, cost-efficient, and multi-user environment that will enhance the productivity of both individual and collaborative research programs. In addition to state-of-the-art equipment and methods, the key personnel of the IBVC provide scientific and technical expertise that greatly benefits the Center investigators in terms of experimental design, execution and interpretation, as well as, by providing a focus for collaborative interactions.