CORE--Tissue Culture and Immunoassay

这是一项关于组织培养和免疫分析核心(TCIC)设施的建议，其目的是为细胞培养维护、细胞和病毒纯化以及免疫学性能提供集中资源，以支持德克萨斯大学医学部(UTMB)题为“呼吸道炎症信号”的计划项目中包括的五个项目。TCIC的具体目标是提供必要的基础设施，以：1.维持 人类细胞系(主要来自上皮源性)；2.制备蔗糖纯化的呼吸道合胞病毒池；3.进行人和小鼠细胞因子和趋化因子的免疫分析。TCIC设施的支持是基于对一些生物试剂、方法学程序和免疫分析的迫切需要，这些都是该计划项目的所有五个项目所共有的。此外，试剂的集中采购和测试的执行将使整个中心的M&O和人员成本降至最低，并将导致分析和实验程序的最佳标准化。拟议的TCIC设施将利用UTMB方案项目调查员目前可用的一些资源，包括占地5,000平方米的儿童健康研究中心(CHRC)实验室。TCIC将实际位于的最先进的实验室设施。TCIC的中心位置设在UTMB园区内，这将促进方案项目不同调查人员之间的沟通，从而更高效、更及时地完成拟议的研究。

This is a proposal for a Tissue Culture and Immunoassay Core (TCIC) facility which purpose is to provide a centralized resource for cell culture maintenance, cellular and viral purification, and immunossay performance, in support of five projects included in the Program Project entitled" Signaling in Airway Inflammation" at University of Texas Medical Branch (UTMB). Specific objectives of the TCIC to provide infrastructure necessary to: 1. Maintain a tissue bank of human cell lines (mostly of epithelial origin); 2. Prepare pools of sucrose-purified respiratory syncytial virus; 3. Perform immunoassays for human and mouse cytokines and chemokines. The support of the TCIC facility is justified based on the critical need for a number of biological reagents, methodological procedures and immunological assays, common to all five projects of the Program Project. In addition, centralized acquisition of reagents and performance of the tests will minimize the cost of M&O and personnel for the whole center and will result in an optimal standardization of assays and experimental procedures. The proposed TCIC facility will take advantage of some resources currently available to the Program Project investigators at UTMB, including the Child Health Research Center (CHRC) laboratory, a 5,000 sq. ft., state-of-art laboratory facility where the TCIC will be physically located. The central location of the TCIC within the UTMB campus, will facilitate the communications among the different investigators of the Program project, resulting in a more efficient and timely completion of the proposed research.