Core B: Anatomy and Behavior Core

摘要-核心B 解剖和非人灵长类行为核心(核心B)将是尤德尔中心的重要组成部分，网址为 埃默里大学。这个核心的主要功能之一将是准备和处理来自 实验动物由该中心项目产生。这项服务将通过标准化的组织学来完成 技术，并将提供必要的信息，以便解释功能性 项目1和项目2下的实验，并将促进所有三个项目之间的结果共享和比较 项目。具体地说，该中心将提供以下服务：(1)免疫组织化学和 评价6-羟色胺治疗动物多巴胺能耗竭程度的显微镜方法 羟基多巴胺(6-OHDA，用于项目1中的小鼠)或1-甲基-4-苯基-1，2，3，6-四氢吡啶(MPTP， 在项目2和3中用于猴子)；(2)使用组织学标记来描绘运动丘脑的核 在啮齿动物(方案1)和猴子(方案2和3)中；(3)记录电极的组织重建 轨迹(项目1和2)；(4)分析病毒感染后Opsins的表达程度和模式 转基因(项目1和2)；(5)检测和数字化重建充满生物细胞的神经元(项目1)； (6)MPTP处理猴脑组织的编目和保存。核心B的第二项主要服务 将用神经毒素MPTP系统地治疗项目2和3中的恒河猴，并评估 帕金森症用标准化的方法治疗引起的帕金森症。这将使我们能够产生动物 在两个项目中的帕金森症水平相似，并将有助于比较 他们。通过提供这些服务，核心将有助于将时间和资源从研究项目中解放出来。 此外，使用标准化的解剖学和行为评估技术将有助于我们保持 始终如一的高质量中心研究。

Summary – Core B The Anatomy and non-human primate behavior Core (Core B) will be an essential part of the Udall Center at Emory University. One of the major functions of this Core will be to prepare and process brain tissue from experimental animals generated by the Center projects. This service will be done with standardized histologic techniques, and will provide information that will be indispensable for the interpretation of the functional experiments under projects 1 and 2, and will facilitate sharing and comparisons of results among all three projects. Specifically, the Core will provide the following services: (1) Use of immunohistochemical and microscopy approaches to evaluate the degree of dopaminergic depletion in animals treated with 6- hydroxydopamine (6-OHDA, used in mice in project 1) or 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, used in monkeys in projects 2 and 3); (2) use of histological markers to delineate nuclei in the motor thalamus in rodents (projects 1) and monkeys (projects 2 and 3); (3) histological reconstructions of recording electrode trajectories (projects 1 and 2); (4) analysis of the degree and pattern of expression of opsins after viral transfection (projects 1 and 2); (5) detection and digital reconstruction of biocytin-filled neurons (project 1); and (6), the cataloguing and storing of brain tissue of MPTP-treated monkeys. A second major service of Core B will be to systemically treat Rhesus monkeys from projects 2 and 3 with the neurotoxin MPTP and evaluate parkinsonism resulting from this treatment with standardized methods. This will allow us to generate animals with similar levels of parkinsonism across both projects, and will facilitate comparisons of data generated by them. By providing these services, the Core will help to free up time and resources from the research projects. In addition, the use of standardized anatomical and behavioral assessment techniques will help us to maintain a consistently high level of quality of Center research.