In Vivo Animal and Human Studies Core

体内动物和人类研究核心的目标是提供一个场所， 人类和动物体内研究所需的资源、设施、新技术和设备。 核心服务于充分整合分子，细胞和器官系统水平的研究， 目前正在该中心的其他核心内进行，并加强基本发现的快速翻译 临床应用。体内动物和人类研究中心提供的主要服务如下： 如下：1.提供多种急性和慢性动物模型以及最先进的体内 技术，以加强快速翻译的基本发现，以生理和临床应用; 2.到 开发类器官和类肠程序，其中这些制剂从粘膜活检中生长， 诱导的多能干细胞（iPSC）可用于研究上皮细胞生物学并提供治疗性免疫应答。 临床干预目标; 3.支持临床研究项目，该项目将提供咨询和 为中心研究者提供设计和统计数据分析方面的技术支持; 4.提供用户 为中心成员提供获取消化系统疾病相关生物标本的友好、一站式服务。以来 自1984年成立以来，体内动物和人类研究核心已经发展到满足中心的需求 investigators.随着时间的推移，临床设施中易于使用的旧技术已经被 中心停止。我们已经简化了我们的动物研究，包括那些经常 由中心研究人员使用。这些研究包括小鼠内窥镜检查、体内细胞和分子成像研究 在小动物中，荧光激活细胞分选（FACS）分析和电穿孔用于直接转移 siRNA进入靶向神经组织或GI器官。类肠和类器官核心的研究进展 使中心研究人员能够对人类iPSCs的体外模型进行研究， 人多能干细胞（hPSC），以及原代人和小鼠组织的三维生长 从胃肠道输送。这些制剂可用于研究人类GI发育、体内平衡 和体外疾病。为了提高基础科学研究成果向临床应用的快速转化， Vivo Studies core花费了大量的精力和资源来升级其基础设施。临床设计 该计划提供有关临床研究设计，受试者规格和采样技术的咨询， 测量和仪器、样本量估计、功效分析以及数据管理， 统计分析生物样本库服务将提供支持，以加强现有的个人GI- 相关的生物库，并开发未来的储存库。该服务还集中、加快和促进 访问GI相关的生物库和利用GI生物标本，以转化基础研究， 人类标本的典范。在此资助期间，体内动物和人类研究核心 该中心60%的成员使用该软件，支持了166份出版物，其中55份是合作出版的。

The objective of the In Vivo Animal and Human Studies core is to provide a venue for focusing human resources, facilities, new technology and equipment necessary for the in vivo study of humans and animals. The core serves to fully integrate investigations at the molecular, cellular, and organ system levels that are currently being performed within other cores of the Center and enhance rapid translation of basic discoveries into clinical application. The major services provided by the In Vivo Animal and Human Studies core are as follows:1. To provide a variety of acute and chronic animal models as well as state-of-the-art in vivo technologies to enhance rapid translation of basic discoveries to physiological and clinical applications; 2. To develop an organoid and enteroid program in which these preparations grown from mucosa biopsies and induced pluripotent stem cells (iPSCs) may be used to study epithelial cell biology and provide therapeutic targets for clinical intervention; 3. To support a clinical study program which will provide consultation and technical support to Center investigators on design and statistical data analysis; and 4. To provide a user friendly, one-step service to access digestive disease-related biospecimens for Center members. Since its inception in 1984, the In Vivo Animal and Human Studies core has evolved to meet the needs of Center investigators. Over time, older techniques which are readily available in clinical facilities have been discontinued by the Center. We have streamlined our animal studies to include ones that are frequently utilized by Center investigators. These include mice endoscopy, in vivo cellular and molecular imaging studies in small animals, fluorescent-activated cell sorting (FACS) analysis, and electroporation for direct transfer of siRNA into targeted neuro-tissue or GI organs. The recent development of the Enteroid and Organoid core enables Center investigators to conduct research on in vitro models of human iPSCs, directed differentiation of human pluripotent stem cells (hPSCs), and 3-dimensional growth of primary human and mouse tissue delivered from the GI tract. These preparations may be used to study human GI development, homeostasis and disease in vitro. To enhance the rapid translation of basic science findings to clinical applications, the In Vivo Studies core has spent much of its efforts and resources to upgrade its infrastructure. The Clinical Design Program provides consultation regarding clinical study design, subject specification and sampling techniques, measurements and instruments, sample size estimation, power analysis, as well as data management and statistical analysis. The Biospecimen Banking service will provide support to enhance existing individual GI- related biobanks and develop future repositories. This service also centralizes, expedites, and facilitates access to GI-related biobanks and utilization of GI biospecimens to translate basic research within the paradigm of human specimens. During this funding period, the In Vivo Animal and Human Studies core has been used by 60% of its Center members, has supported 166 publications, with 55 being collaborative.