Core C: Pathology, Biospecimen and Immune Profiling

病理学、生物标本和免疫分析核心将选择、储存并提供集中的 对RESPOND队列中收集的生物标本进行病理分析，确保 生物样本分析物可用于所有下游分析。核心将主要支持项目2， 图3和图4所示的生物样本、生物样本分析物以及病理和图像数据库。主要功能 的核心是病理学注释和库福尔马林固定石蜡包埋（FFPE）前列腺肿瘤 在RESPOND（项目2、3和4）中，为大约3，000名患者提供组织，分离并定量核酸 从这些组织（项目3）和从7,500个唾液样本（项目2）中提取的具有适当质量的酸 控制/质量保证措施，并测试和实施图像分析技术， 原位肿瘤相关免疫浸润（项目4）。核心的活动将集中在约翰霍普金斯大学 大学（JHU），并将利用众多正在进行的生物标本库工作和前列腺肿瘤 JHU的组织分析，包括JHU前列腺癌孢子生物标本/病理学核心和 CDMRP前列腺癌生物储存网络。核心小组将由两名泌尿病理学家领导， Tamara Lotan MD（PI，JHU）和Angelo M. De Marzo MD PhD（co-I，JHU）以及两名科学家， 与核心相关的特定生物标本分析的广泛专业知识，Karen Sfanos博士（co-I，JHU）和 克里斯·海曼（南加州大学共同I）。除了对3个项目的广泛支持外，核心的生物库和数据库将 为所有未来的研究工作提供一个持续的资源，利用响应队列及其 生物标本

The Pathology, Biospecimen and Immune Profiling Core will select, bank, and provide centralized pathologic analysis for biospecimens collected in the RESPOND cohort, ensuring that uniformly high quality biospecimen analytes are available for all downstream analyses. The Core will principally support Projects 2, 3 and 4 with biospecimens, biospecimen analytes and pathologic and image databases. The major functions of the Core are to pathologically annotate and bank formalin fixed paraffin embedded (FFPE) prostate tumor tissue for approximately 3,000 patients within RESPOND (Projects 2, 3 and 4), isolate and quantify nucleic acids from these tissues (Project 3) and from 7,500 saliva samples (Project 2) with appropriate quality control/quality assurance measures, and test and implement image analysis technologies for interrogating tumor-associated immune infiltrates in situ (Project 4). The Core’s activities will be centered at Johns Hopkins University (JHU) and will leverage the numerous ongoing biospecimen banking efforts and prostate tumor tissue analyses at JHU, including the JHU Prostate Cancer SPORE Biospecimen/Pathology Core and the CDMRP Prostate Cancer Biorepository Network (PCBN). The core will be led by two urologic pathologists, Tamara Lotan MD (PI, JHU) and Angelo M. De Marzo MD PhD (co-I, JHU) as well as two scientists with extensive expertise for specific biospecimen analyses relevant to the Core, Karen Sfanos PhD (co-I, JHU) and Chris Haiman (co-I, USC). In addition to extensive support for 3 Projects, the Core’s biobank and database will provide an ongoing resource for all future research endeavors utilizing the RESPOND cohort and its biospecimens.