Animal Shared Resource

项目总结-动物共享资源 Wistar癌症中心经营AAALAC认证的动物共享资源（ASR）服务教师 所有三个科学项目。专家管理，饲养，保管和兽医工作人员保持 ASR，包括改良的生物安全2级（BSL 2）屏障，具体目标如下： ·提供实验室动物医学和饲养服务，日常动物采购，动物健康 监测、兽医护理、库存监测、检疫住房、日常技术支持和 为所有在研究中使用动物的癌症中心研究人员提供技术培训。 ·维持NSG（NOD.Cg-Prkdcscid Il 2 rgtm 1 Wjl/SzJ）繁殖集落用于产生免疫缺陷的 小鼠 ·通过我们的国际合作，为转基因和转基因小鼠的生产提供专业知识， 与福克斯蔡斯癌症中心的转基因小鼠设施进行机构合作。 ·提供全职兽医病理学家大卫加利克，D.V.M.的服务。 ·管理机构动物护理和使用委员会（IACUC）强制要求的新员工入职培训， IACUC程序培训以及新研究人员的无菌技术培训。 ·拥有一套用于体内成像的套件，包括PerkinElmer IVIS Spectrum µCT成像系统。 建筑工程已经完成，新的、最先进的动物园于2013年11月开放。配备 机架空间容纳6，000多个无菌、一次性和单独通风的鼠笼，该品牌 一个新的，13,570国家科学基金会，饲养场采用技术先进的，一次性的，单独通风的笼子 由新的HVAC系统支持。自2013年以来，超过1400万美元的机构投资已经 分配给ASR，以完成癌症中心（CC）的使命和研究目标。这一重大 资本分配允许建设和装修新的动物园，包括功能，提高 为癌症中心的研究人员提供动物护理的所有领域。门禁系统包括身份证刷卡，生物识别 指纹扫描和监控摄像头拘留室配备了新的通风架。进一步 投资包括隔离栅栏，这是独立的物种，并允许最大的灵活性， 为密封和隔离提供行业标准。在项目期间， 新的CC成员推动了小鼠动物模型使用量的47%增长。另一种动物 2016年聘请了护理员，以满足日益增长的服务需求。简化日常运作 在ASR中，新的IACUC方案和动物设施管理系统将于2018年纳入。 总之，ASR继续发挥重要作用，支持所有三个计划的科学组合， 癌症中心，切实有助于关键的多学科科学进步。

PROJECT SUMMARY – ANIMAL SHARED RESOURCE The Wistar Cancer Center operates an AAALAC—accredited Animal Shared Resource (ASR) serving faculty from all three scientific Programs. Expert management, husbandry, custodial and veterinary staff maintain the ASR, including a modified Biosafety Level 2 (BSL2) barrier, with the following specific aims: · Provide service in laboratory animal medicine and husbandry, routine animal procurement, animal health surveillance, veterinary care, inventory monitoring, quarantine housing, routine technical support and technical training for all Cancer Center investigators using animals in their research. · Maintain an NSG (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) breeding colony for the generation of immunodeficient mice. · Provide access to expertise for the generation of transgenic and genetically modified mice through our inter- institutional collaboration with the transgenic mouse facility at Fox Chase Cancer Center. · Provide access to the services of a full time veterinary pathologist, David Garlick, D.V.M. · Administer Institutional Animal Care and Use Committee (IACUC) mandated new employee orientation and IACUC procedures training as well as aseptic technique training for new researchers. · House a suite for in vivo imaging, including a PerkinElmer IVIS Spectrum µCT Imaging System. Construction was completed, and the new, state-of-the-art vivarium opened in November 2013. Equipped with rack space to house more than 6,000 sterile, disposable, and individually-ventilated mouse cages, the brand new, 13,570 NSF, vivarium employs technologically-advanced, disposable, and individually-ventilated cages supported by a new HVAC system. Since 2013, an institutional investment of more than $14 million has been allocated to the ASR to fulfill the mission and research objectives of the Cancer Center (CC). This significant capital allocation allowed for the construction and fit-out of the new vivarium, including features which enhance all areas of animal care for Cancer Center investigators. Access controls include ID card swipe, biometric fingerprint scan, and security cameras. Holding rooms are equipped with new ventilated racks. Further investments include isolation cubicles, which are species independent and allow for maximum flexibility while providing the industry standard for containment and isolation. During the project period, the recruitment of 10 new CC members has driven a 47% increase in the use of murine animal models. An additional animal caretaker was hired in 2016 to meet the increasing demand for services. To streamline day-to-day operations of the ASR, a new IACUC protocol and Animal Facility Management system is being incorporated in 2018. Together, the ASR continues to play an essential role to support the scientific portfolio of all three Programs in the Cancer Center, tangibly contributing to key multidisciplinary scientific advances.