Organ Specific Project - Kidney

项目摘要 肾脏OSP的目标是通过开发 基于超高含量质谱（MS）的3维（3-D）成像流水线，以表征 不同细胞类型和细胞外基质的分子特征（脂质、代谢物和蛋白质） 在完整的人肾脏中以高分辨率检测组分。这个独特的平台获取的成像数据 将与通过基于坐标的对齐获得的人体肾脏的3-D结构对齐并融合 MRI、CT和组织化学成像。这一技术管道将被开发为兼容 通过HuBMAP生成的其他分子询问管道， 正常人肾脏的多维三维分子图谱。

Project Summary The goal of the Kidney OSP is to address the limitation in current knowledge of kidney biology by developing an ultra-high content mass spectrometry (MS)-based 3-dimensional (3-D) imaging pipeline to characterize the molecular signatures (lipids, metabolites and proteins) of different cell types and extracellular matrix components at high resolution in the intact human kidney. The imaging data acquired by this unique platform will be aligned and fused with the 3-D structure of the human kidney obtained by coordinate-based alignment with MRI, CT and histochemical based images. This technological pipeline will be developed to be compatible with other molecular interrogation pipelines generated through HuBMAP to create a comprehensive multidimensional 3-D molecular atlas of the normal human kidney.