Macromolecular Crystallography

摘要：高分子结晶学核心 大分子结晶学(MX)核心的目的是提供访问和支持使用， 世界一流的MX光束线。通过该核心支持两条MX光束线，第一条是高度 自动高分子结晶光束线(AMX)(17ID-1)，一种可调、高通量微束，其中 我们把重点放在实验的自动化上。第二条光束线，前沿微聚焦 大分子晶体学(FMX)(ID17-2)提供了尺寸可从1可调的高通量x射线束 到10微米，以及支持这种资源使用的软件和硬件。 资源的成功依赖于实验期间的访问、支持以及对用户使用的培训 我们的光束线。我们通过由国家安全委员会运行的同行审查程序确保基于优点的访问 同步辐射光源II(NSLS-II)。为了满足社会的需要，我们开发了多路接入 允许以科学为导向，优先接触最多的主要调查人员的类型。这个 实验的审查由独立的同行审查小组(PRP)执行，他们生成记录的 对用户提交的每种访问模式的每个项目进行排名和评论。此外，《审查制度》还规定 定期反馈光束线的性能和未来方向。 我们首先通过互动的方式为资源的用户提供支持，讨论他们的实验计划，并 其次，对科学家及其同事进行使用仪器的培训。我们实现了可靠的运行 通过为我们的仪器应用性能标准，并通过执行预定的预防措施 维修。每个实验都被分配了一个“当地联系人”，这是培训和支持的第一个联系人， 谁负责每一次实验前、中、后的联络。我们有一支由7人组成的博士生团队 结晶学家，以及几个交叉培训的操作AMX和FMX。以帮助有效地使用这两个 我们已经为实验开发了通用的控制软件和通用的硬件 有可能。这提供了多种好处：三组不同的员工只需要知道一套控制措施- 光束线科学家、来访的用户以及设备支持工程师和工作人员。 我们有计划继续开发处于技术前沿的NSLS-II MX光束线 可用。发展分为三个领域：提供x射线束的光学部件， 用于进行衍射实验的硬件和软件，以及我们数据的持续改进- 分析软件和硬件。光束线仪器的开发包括部署多层 增加亮度的单色仪，增加产量的新样品输送格式，以及新的管道 以改进数据处理。我们的仪器将得到改进，以便更有效地处理 微晶体和通过各种方法将样品输送到光束线中：注射器、高密度微米 支撑物和样品的现场检验。

Abstract: Macromolecular Crystallography Core The aim of the macromolecular crystallography (MX) core is to provide access to, and support for the use of, world-class beamlines for MX. There are two MX beamlines supported through this core, the first is the Highly Automated Macromolecular Crystallography beamline (AMX) (17ID-1), a tunable, high flux microbeam where we have placed an emphasis on automation of the experiments. The second beamline, Frontier Microfocusing Macromolecular Crystallography (FMX) (ID17-2) offers a high-flux x-ray beam with dimensions tunable from 1 to 10 micrometers, and the software and hardware to support the use of this resource. The success of the resources depends on access, support during experiments, and training of the users in the use of our beamlines. We assure merit-based access through a peer-reviewed process run by the National Synchrotron Light Source II (NSLS-II). To meet the needs of the community we have developed multiple access types that allow science-driven prioritization of access to the largest number of principal investigators. The review of experiments is performed by the independent Peer Review Panel (PRP), who generate a documented ranking and critique for every project submitted by users for each access mode. In addition, the PRP provide regular feedback on the performance and future direction of the beamlines. We provide support for the users of our resource firstly by interaction to discuss their experimental plan, and secondly by training of the scientists and their co-workers to use the instruments. We achieve reliable operation by applying performance standards for our instrumentation, and by performing scheduled preventive maintenance. Each experiment is assigned a “local contact,” the first point of contact for training and support, who is responsible for liaison before, during, and after each experiment. We have a team of seven PhD-trained crystallographers, and several are cross-trained to operate both AMX and FMX. To aid efficient use of the two beamlines we have developed common controls software for the experiments and common hardware when possible. This provides multiple benefits: three different groups of workers need know only one set of controls – the beamline scientists, the visiting users, and the equipment-support engineers and staff. We have a plan to continue the development of the NSLS-II MX beamlines at the leading edge of the technology available. The developments fall into three areas: the optical components that provide the x-ray beams, the hardware and software for performing diffraction experiments, and continued improvements of our data- analysis software and hardware. Beamline instrumentation developments include deploying a multi-layer monochromator to increase brightness, new sample-delivery formats to increase throughput, and new pipelines to improve data handling. Our instrumentation will be improved to allow for more efficient handling of microcrystals and delivery of samples into the beamline by a variety of methods: injectors, high density micro supports and in-situ examination of samples.