Microbiome Research Core

项目摘要-微生物核心 微生物群落和分离株的特征是实现科学的 拟建的新陈代谢变革性研究中心的目标。微生物组核心将 提供致力于微生物组研究和培训的独特服务。核心的目标是支持 全面的微生物群落和分离鉴定流水线，在鉴定时 将产生解释宿主-微生物共生的可测试假说。我们将通过以下方式实现这一目标 实现以下特定目标(SA)。SA1.提供和维护最先进的技术和 以微生物群落和分离物为特征的高通量管道的程序。SA2.提供 在微生物组研究方面的专业知识，从实验设计和样本收集到数据分析和 生物信息学。SA3.通过专家咨询支持和发展微生物组研究和传播， 并通过与科布雷调查人员和普通公众进行外联活动。它的新奇之处在于 提议的核心是组合多个模块，研究人员可以在管道沿线的任何点插入这些模块或 访问完整的管道，以确定分离株或微生物群落的特征。微生物组核心是一种 一期中心支持的以基础研究为重点的研究项目的基本组成部分 转化为新陈代谢中的微生物-宿主共生。核心提供所需的专业知识、设备和软件 用于生物、分子和生物信息学分析。核心维护的关键设备包括 微生物生物反应器，允许用户设置类似于宿主肠道的实验条件，并扰乱 识别关键微生物过程的系统。Illumina MiSeq和Nanopore奴才将提供 分析分子基础的测序能力。与INBRE生物信息学合作 微生物组核心将提供微生物组研究所需的生物信息学算法方面的专门知识。

Project Summary - Microbiome Core Characterization of microbial communities and isolates is a key element necessary to achieve the scientific objectives of the proposed Center in Transformative Research in Metabolism. The Microbiome Core will provide a unique service dedicated to microbiome research and training. The goal of the Core is to support a comprehensive pipeline for characterization of microbial communities and isolates, which when characterized will generate testable hypotheses explaining host-microbiome symbiosis. We will achieve this goal by accomplishing the following Specific Aims (SA). SA1. Provide and maintain state-of-the-art technologies and procedures for a high throughput pipeline that characterizes microbial communities and isolates. SA2. Provide expertise in microbiome research, from experimental design and sample collection to data analysis and bioinformatics. SA3. Support and grow microbiome research and dissemination through expert consultation, and by engaging in outreach events with COBRE investigators and the general public. The novelty of this proposed core is to combine multiple modules that researchers can plug into at any point along the pipeline or access the full pipeline to characterize either isolates or microbial communities. The Microbiome Core is an essential component of the Research Projects supported by the Phase 1 center that focus on basic research into microbe-host symbiosis in metabolism. The Core provides the expertise, equipment, and software required for organismal, molecular, and bioinformatic analysis. Key equipment maintained by the Core includes a microbial bioreactor that allows users to set up experimental conditions similar to host guts and perturb the system to identify key microbial processes. An Illumina MiSeq and Nanopore MinION will provide the sequencing power to analyze the molecular underpinnings. In collaboration with the INBRE Bioinformatics Core, the Microbiome Core will provide expertise on bioinformatic algorithms needed for microbiome research.