Method validation for the analysis of cannabinoids in animal specimen and hemp-based animal feed products

项目摘要 本应用的目的是开发和验证大麻中大麻类化合物的分析方法。 在衍生食品和动物标本中也是如此。2014年的农场法案使种植合法化 和生产工业大麻(含0.3%四氢大麻酚的大麻)和 工业大麻被列为DEA附表I药物。最近，2018年农场法案已经 允许增加对大麻种植的兴趣，作为一种新的农产品，在某些情况下 规定。大麻植物及其副产品，被认为具有营养和潜在的 在牲畜中用作饲料来源时的治疗价值。许多商业上可以买到的大麻 食用油和饲料产品在市场上随处可见，但对其大麻素知之甚少。 内容。此外，有必要在动物标本中开发一种大麻素筛查。 已知口服给药后它们在牛体内的分布以及所需的时间 可食用组织中的枯竭。还需要有一项诊断测试在 毒理实验室的宠物经常被大麻衍生的产品陶醉： 在这种情况下，血浆和尿液将是检测接触THC的首选样本。结果是 将包括：1)扩大FDA调查动物性食品或药品的能力 关切，2)提高了应对大规模动物性食品/饲料紧急事件的能力； 3)提高联邦和州动物监测的质量和标准化 食物和饲料。我们已经有了量化油和大麻中的大麻类化合物的经验 补充剂，并已开始研究开发牛血浆中的方法。

Project Summary The purpose of this application is develop and validate the analysis of cannabinoids in hemp- derived food products as well as in animal specimen. The 2014 Farm Bill legalized the cultivation and production of industrial hemp (Cannabis sativa containing < 0.3%Tetrahydrocannabinol) and the removal of industrial hemp as DEA Schedule I drug. More recently, the 2018 Farm Bill has allowed for increased interest in hemp cultivation as a novel agricultural commodity under certain provisions. Hemp plants and their by-products, are considered to have nutritional and potentially therapeutic value when used as a feed source in livestock. Many commercially available hemp oils and feed product are available on the market with little information of their cannabinoids content. In addition, there is a need to develop a cannabinoid screen in animal specimen as little is known about their distribution in cattle following oral administration and the time required for depletion in edible tissues. There is also a need to have a diagnostic test available in the toxicology laboratory as pets are regularly intoxicated with cannabis-derived products: in this case, plasma and urine would be specimen of choice to detect exposure to THC. The outcomes will include: 1) the broadening capacity of the FDA to investigate animal food or drug related concerns, 2) an Increased capacity to respond to large-scale animal food/feed emergency events, 3) the improvement in the quality and standardization of Federal and State surveillance of animal food and feed. We already have experience in quantifying cannabinoids in oil and hemp-based supplements and have started to work on developing the method in bovine plasma.