Core C: Customized Physiology and Imaging Core

项目总结 拟建的佛蒙特州心血管中心的新定制成像和生理学核心 大脑健康科布雷将基于神经科学成像核心从以前的 科布雷(2016年完成)。它将满足尖端成像调查的新需求 由项目主管(PD)在新提议的Cobre中进行。我们将采用所需的采购 技术和翻新，以适应其运营。定制的生理学和成像核心将 旨在为活体样本提供最先进的成像能力，包括活体、组织和细胞 制剂，以及固定样品。新的心血管和大脑健康科布雷将涉及 在活体成像和电生理学以及利用遗传编码指标方面进行广泛的培训 以及由项目主管提供的光学驱动的探测器。这一核心将有高水平的实验前和实验后 托德·克莱森、格兰特·亨尼格博士和汤姆·赫普纳博士提供的技术和分析支持。的新时代 高分辨率显微镜需要对清醒的动物进行成像的灵活性，这些动物可能正在经历特定的 任务，并在从毫秒到几周的大范围内以3个维度成像。至 要做到这一点，这个核心将建议和培训调查人员使用不同类型的最先进的高科技 空间和时间分辨率显微镜、电生理技术和3D打印仪器 设计和制造以响应不断变化的研究需求，以及在图像的使用和开发中 和电生理分析软件。这个核心的目标是为调查人员配备 成像/电生理、制造和分析技能，以便他们能够适应 解决复杂问题。预计这一核心将极大地增强Cobre的竞争力 项目主任以及心血管和脑健康研究的全校调查人员。此核心 独一无二的是，在高度成功的核心人员的支持下，警局和其他调查人员将 掌握执行实验、修改成像模式、制造特定项目的技能 设备，并执行高级图像分析。

PROJECT SUMMARY The new Customized Imaging and Physiology Core of the proposed Vermont Center for Cardiovascular and Brain Health COBRE will be based upon transformation of a Neuroscience Imaging Core from a previous COBRE (completed in 2016). It will meet the new needs of cutting-edge imaging investigation that will be pursued by Project Directors (PDs) in the newly proposed COBRE. We will employ acquisition of needed technology and renovation to accommodate its operation. The Customized Physiology and Imaging Core will be designed to provide state-of-the-art imaging capabilities for live samples, including intravital, tissue and cell preparations, as well as fixed samples. The new Cardiovascular and Brain Health COBRE will involve extensive training in in vivo imaging and electrophysiology, and exploitation of genetically-encoded indicators and optically-actuated probes by Project Directors. This Core will have high level pre- and post-experiment technical and analytical support from Todd Clason, Dr. Grant Hennig and Dr. Tom Heppner. The new era of high-resolution microscopy requires the flexibility to image in awake animals that may be undergoing specific tasks, and to image in 3 dimensions over a wide range of time periods from milliseconds to weeks. To accomplish this, this Core will advise and train investigators in the use of different types of state-of-art high spatial and temporal resolution microscopy, electrophysiological techniques, and 3D printing for instrument design and fabrication to respond to changing research needs, as well as in use and development of image and electrophysiological analysis software. The goal of this Core is to equip the investigators with imaging/electrophysiological, fabrication and analysis skills so that they are capable of adapting approaches to solve complex problems. It is anticipated that this Core will greatly enhance the competitiveness of COBRE Project Directors as well as campus-wide investigators in cardiovascular and brain health research. This Core is unique in that PDs and other investigators, with support from highly accomplished Core personnel, will acquire the skills to perform the experiments, modify imaging modalities, to fabricate project-specific equipment, and to perform high level image analysis.