A novel method for improved separation of metagenomic samples for preservation and identification.

摘要 所有基于微生物组的研究的成功高度依赖于有效的样品纯化 目标是感兴趣的生物。这些元基因组样本的个体组织成分的特征 在很大程度上依赖于分离个体系统发育成分的能力，例如将人类 从微生物组分中分离细胞，同时防止样品丢失或降解。这是第一阶段的STTR项目 旨在显著改进元基因组学/环境样本(例如人类)的DNA纯化方法 和细菌)使用一种正在申请专利的简单且廉价的纯化方法，旨在有效地 分离和保存宿主和微生物的DNA。这种方法将确保所有生物的DNA都被保存下来 与现有DNA使用的方案不同的是，在初始分离步骤中不会出现“牺牲”或丢失 净化试剂盒制造商。结果应产生更高质量的DNA序列数据(即更高 测序覆盖范围和更高的灵敏度)，从而提供健壮的信息内容，从而导致更高的 对生物特征的信心。 锡拉丘兹生物实验室公司(SBL)已与法医和国家安全领域的科学家合作 锡拉丘兹大学科学研究所(FNSSI)进一步改进这种新型DNA并将其商业化 提纯方法。该第一阶段项目有三个目标，旨在建立一个概念验证 方法开发可现场应用的基因组DNA纯化模块试剂盒。我们的 元基因组DNA纯化试剂盒也适用于人类微生物组研究以外的领域 市场，包括与犯罪和犯罪有关的生物样本的法医鉴定和地理定位 与智能相关的活动，基于DNA的生态研究，基于植物和微生物的地球资源和 国防/国土安全工作(要求从环境样本中提纯DNA)。

Abstract The success of all microbiome-based studies are highly dependent on effective sample purification of the target organisms of interest. Characterizing individual organismal components of these metagenomic samples relies heavily upon the ability to separate the individual phylogenetic components, such as removing human cells from the microbiological fraction while preventing samples loss or degradation. This Phase I STTR project aims to significantly advance DNA purification methods of metagenomics / environmental samples (e.g. human and bacteria) using a patent pending simple and inexpensive purification approach aimed at effectively separating and preserving host and microbial DNA. This method will ensure all organismal DNA is preserved (i.e. not “sacrificed” or loss) during initial separation steps, unlike protocols employed by existing DNA purification kit manufacturers. Outcomes should result in higher quality DNA sequence data (i.e. higher sequencing coverage and higher sensitivity) thus providing robust information content, leading to higher confidence in the organismal characterization. Syracuse Biolabs, Inc. (SBL), has teamed with scientists in the Forensic and National Security Sciences Institute (FNSSI) at Syracuse University to further improve and commercialize this novel DNA purification approach. This Phase I project has three aims directed to establish a proof-of-concept of the method leading to initial kit development for fieldable DNA purification modules of metagenomic samples. Our metagenomics DNA purification kit is also applicable to fields outside of the human microbiome research market, including forensic identification and geolocation of biological samples relevant to criminal and intelligence related activities, DNA based ecological research, plant and microbial based geosourcing and defense/homeland security efforts (requiring DNA purification from environmental samples).