Microbiome and Transcriptomics Core (MTC)

摘要（微生物组和转录组学核心- MTC） 目前在微生物发病机理和免疫领域的研究需要了解全细胞和 对宿主和病原体的感染的系统范围的反应。如果没有最先进的专业知识 技术和分析工具，收集这些信息，不能取得进展的速度 保持在科学发现的最前沿所需要的。微生物组和转录组学核心 (MTC)将发挥不可或缺的作用，确保我们的COBRE研究人员能够正确收集微生物组， 使用最先进的技术和方法，以最高的严谨程度获得基因表达数据。的 MTC将是现有的OUHSC分子生物学和细胞计数研究实验室的扩展 （LMBCR），一个建立和管理良好的大学核心实验室。MTC将雇用来自 在LMBCR和INBRE生物信息学核心内，在重点领域（下一代）拥有丰富的经验 测序方法，包括微生物组和RNAseq，以及生物信息学） 调查项目。MTC将提供全细胞/系统范围的基因表达分析， 建立了下一代测序和转录组学技术以及适当的生物信息学 方法。MTC对这些应用程序的支持将包括项目前咨询， 样品制备和RNA分离方案，湿台样品处理，生物信息学分析和 后期项目数据存储、手稿准备，并协助提供未来资助所需的详细信息 应用.

ABSTRACT (Microbiome and Transcriptomics Core - MTC) Current studies in the area of microbial pathogenesis and immunity require knowledge of whole cell and system wide responses to infection of both the host and pathogen. Without proper expertise in state of the art technology and analysis tools necessary to gather this information, progress cannot be made at the pace required for remaining at the forefront of scientific discovery. The Microbiome and Transcriptomics Core (MTC) will serve an integral role in ensuring that our COBRE researchers can properly collect microbiome and gene expression data at the highest level of rigor using the most advanced technology and methods. The MTC will be an extension of the existing OUHSC Laboratory for Molecular Biology and Cytometry Research (LMBCR), an established and well-managed university Core laboratory. The MTC will employ personnel from within the LMBCR and INBRE Bioinformatics Core that have extensive experience in the focus areas (next gen sequencing methods, including microbiome and RNAseq, and bioinformatics) required for the Junior Investigator projects. The MTC will provide whole cell/system wide analysis of gene expression using established next generation sequencing and transcriptomics technology and appropriate bioinformatics approaches. Support for these applications by the MTC will include pre-project consultation, assistance with sample preparation and RNA isolation protocols, wet-bench sample processing, bioinformatics analysis and post project data deposition, manuscript preparation, and aid with details necessary for future grant applications.