Small Animal Imaging Core

摘要-小动物成像核心 在COBRE第一阶段下建立的小动物成像核心（SAIC） 俄克拉荷马州大学健康科学中心（OUSHC）提供最先进的 用于评价肿瘤生长、进展、转移的小动物成像模式， 对治疗的反应。国家工商行政管理总局的职能包括： 设计涉及分子成像的体外和体内研究。工商总局工作人员提供 他们的专业知识，以COBRE首席初级调查员（PJIs），试点项目初级 研究者（PPI），以及来自OUHSC和邻近机构的其他研究者， 需要非侵入性分子成像的动物研究;提供使用 成像设备;以及随后的数据收集和分析。SAIC还提供 协助优化成像和其他成像前和成像后外科手术。在 此外，SAIC还提供先进的高内涵活细胞成像。此外，SAIC还开发了一种 具有注释的分子标签的人和鼠癌细胞系的细胞库， 表征了使用这些细胞系消除了实验结果的可变性， 保持僵硬最后，SAIC员工每年都要接受软件更新和其他培训。 与成像相关的技术，使他们跟上成像的进展。 在第一阶段COBRE资助期间，4名PJIs，2名PPI和89名其他研究人员 接受过使用各种设备的服务和/或培训，并已获得SAIC 进行研究的设施。这导致获得了重要的科学数据， 推进研究项目，导致会议报告，同行评议的出版物， 并提交赠款申请。最后，上汽集团继续扩大设施， 新设备使研究人员能够进行尖端研究。 在第二阶段，工商总局计划继续培训和提供援助，以新的PJI， PPIS和其他研究人员成功地进行了动物研究， 严谨性和可重复性。为了实现这些目标，上汽集团确定了三个具体目标 - 1）提供服务，协助和咨询，设计体外和体内研究相关 分子成像; 2）提供标记的细胞用于监测基因表达，细胞运输， 以及体外和体内模型中的疾病进展; 3）有助于高通量和高- 活细胞的内容物成像以及肿瘤异种移植物和其它组织的成像。 SAIC服务的持续可用性将允许PJIs，PPI和其他调查人员 为提交拨款申请和获得研究资金提供初步数据。

Abstract - Small Animal Imaging Core The Small Animal Imaging Core (SAIC) established under the Phase I COBRE at the University of Oklahoma Health Sciences Center (OUSHC) provides access to state of the art small animal imaging modalities for evaluating tumor growth, progression, metastasis, and response to therapy. The functions of the SAIC include providing assistance, and consultation in designing in vitro and in vivo studies that involve molecular imaging. The SAIC staff's provide their expertise to the COBRE principal junior investigators (PJIs), pilot project junior investigators (PPIs), and other investigators from OUHSC and neighboring institutions with animal studies that require non-invasive molecular imaging; provide training in the use of the imaging equipment's; and subsequent collection and analysis of data. SAIC also provides assistance in optimizing imaging and other pre- and post-imaging surgical procedures. In addition, SAIC provides advanced high-content live cell imaging. Further, SAIC has developed a cell bank of human and murine cancer cell lines with molecular tags that are annotated and characterized. Using these cell lines eliminates variability in the experimental results and maintain rigor. Finally, SAIC staff's undergo annual training for software updates and other imaging-related techniques to keep them abreast of advances in imaging. During the Phase I COBRE funding period, 4 PJIs, 2 PPIs, and 89 other investigators have received service and/or training in using various equipment's and have accessed the SAIC facility for conducting their research. This has led in obtaining significant scientific data and advancing research projects resulting in conference presentations, peer-reviewed publications, and grant application submissions. Finally, SAIC has continued to expand the facility by adding new equipment's thus enabling investigators in conducting cutting-edge research. In the Phase II period, the SAIC plans continuing to train and offer assistance to new PJIs, PPISs, and other investigators in successfully conducting animal studies with ease and have rigor and reproducibility. For achieving these objectives, SAIC has identified three specific aims – 1) provide service, assistance, and consultation in designing in vitro and in vivo studies related to molecular imaging; 2) provide labeled cells for monitoring gene expression, cell trafficking, and disease progression in in vitro and in vivo models; 3) Assist in high-throughput and high- content imaging of live cells and in imaging of tumor xenografts and other tissues. Continued availability of SAIC services will allow PJIs, PPIs, and other investigators in generating preliminary data for submitting grant applications and obtaining research funding.