Employing smart underwear to measure gut microbial hydrogen sulfide production

项目摘要/摘要 循环气体传递器H₂S是由严格调控的人体酶和 由人类微生物区系以依赖饮食的方式外源产生的。外源H_2S的产生 人体肠道微生物区系通过调节全身硫化氢的生物利用度来影响人类健康。肠胃过多 微生物产生硫化氢被认为与结直肠癌和溃疡性结直肠癌的病因学有关 结肠炎。我们对肠道微生物产生的H₂S如何影响人类健康的了解还不完全 因为目前的测量技术不精确，具有侵入性，需要生物标本收集，并且有 有限的时间分辨率。在这项建议中，我们的目标是通过开展一项研究来解决这一差距- 可长期实时测量肠道微生物H₂S的等级可穿戴智能内衣装置 在气态直肠流出物(排气)中产生。排气是测量肠道微生物硫化氢的未开发来源 制作。我们已经演示了智能内衣v1原型可以精确测量 体外生理相关浓度的硫化氢。当人类穿着时，Smart内衣v1 Prototype以极好的信噪比检测排气，并能够估计硫化氢的浓度 在每一个排气口里。在R21阶段，我们建议为以下产品开发第二版智能内衣原型 通过微型化、软件开发和柔性材料的结合进行临床试验。此外, 我们建议进行用户体验试验，以衡量用户对Smart的接受程度并测试Smart 人类穿着时的内衣装置。在R33阶段，我们将使用智能内衣v2原型 在R21阶段开发，以测试智能内衣设备可以检测到饮食诱导的假设 对比肠道的随机对照喂养试验中肠道微生物硫化氢产生的变化 调节膳食半胱氨酸摄入量生产微生物H-₂S拟议目标的完成将导致 到第一个能够长期实时测量肠道微生物硫化氢产生的可穿戴设备。 这将有助于设计个性化的饮食干预措施，合理调节肠道微生物H₂S 未来寻求解开肠道微生物硫化氢之间复杂相互作用的研究成果和效益 生产和疾病。

PROJECT SUMMARY/ABSTRACT The circulating gasotransmitter H₂S is endogenously produced by tightly-regulated human enzymes and exogenously produced in a diet-dependent manner by the human microbiota. Exogenous H2S production by the human gut microbiota influences human health by modulating systemic H2S bioavailability. Excessive gut microbial H2S production is hypothesized to be involved in the etiology of colorectal cancer and Ulcerative Colitis. Our understanding of how gut microbially produced H₂S affects human health remains incomplete because current measurement techniques are imprecise, invasive, require biospecimen collection, and have limited temporal resolution. In this proposal, our objective is to address this gap by developing a research- grade wearable Smart Underwear device that enables long-term real time measurements of gut microbial H₂S production in gaseous rectal effluent (flatus). Flatus are an untapped source for measuring gut microbial H2S production. We have demonstrated that the Smart Underwear v1 prototype can precisely measure physiologically-relevant concentrations of H2S in vitro. When worn by humans, the Smart Underwear v1 prototype detects flatus with an excellent signal-to-noise ratio and is able to estimate the concentration of H2S in each flatus. In the R21 phase, we propose to develop a second revision Smart Underwear prototype for clinical trials through miniaturization, software development, and incorporation of flexible materials. In addition, we propose to conduct a user experience trial to gauge the user acceptance and test the reliability of the Smart Underwear device when worn by humans. In the R33 phase, we will use the Smart Underwear v2 prototype developed in the R21 phase to test the hypothesis that the Smart Underwear device can detect diet-induced alterations in gut microbial H2S production using a randomized controlled feeding trial that contrasts gut microbial H₂S production by modulating dietary cysteine intake. The completion of the proposed goals will lead to the first wearable device that enables long-term real time measurements of gut microbial H2S production. This will facilitate the design of personalized dietary interventions to rationally modulate gut microbial H₂S production and benefit future studies seeking to untangle the complex interactions between gut microbial H2S production and disease.