Flow & Image Cytometry Shared Resource

项目摘要/摘要 FICSR的使命是提供当代先进的 流式细胞仪分析和分类服务，以及在细胞和亚细胞水平的成像服务 在成本效益高、用户友好和科学严谨的环境中解决这些问题。的一个主要战略目标 资源是提供各种劳动密集型、高度专业化的定制方法开发服务 技巧。方法论的发展往往是经验性的，不容易从 外部消息来源。在本报告所述期间，FICSR获得了新的仪器，增加了能力， 建立新服务、扩展教育项目并参与共享资源咨询 委员会制定战略计划，使我们能够继续满足快速发展的研究 CCSG成员的需求。FICSR总共为134名罗斯韦尔用户提供服务，其中111名(93%)是CCSG 会员。使用FICSR的研究调查人员有三种类型。1级用户是指 精通技术，了解仪器的性能。对于这些调查人员来说，大多数 我们的用户基础、资源提供监督和咨询。2级用户完全依赖于 资源工作人员的专门知识，以提供样品准备、数据采集、数据分析和 释义。3级用户包括不熟悉流式细胞术和/或图像细胞术的调查人员，或刚刚接触 开始考虑这项技术的新应用，以解决他们的研究问题。在这里， 资源工作人员可能会进行试点实验，以确定什么是有用的方法。具体目标 FICSR的内容包括：1)为Roswell Park CCSG成员提供具有成本效益的流程和图像 根据遵循法规的质量保证计划进行维护的细胞学技术 机构指南，并满足其研究计划的功能要求；2)教育罗斯韦尔 PARK CCSG成员介绍流式细胞仪和图像细胞仪技术的新发展、应用和 与其具体研究领域有关的执行情况；3)加强方案间和方案内 通过提供教育和用户友好的环境进行协作，FICSR人员在其中提供帮助 用户具有与用户的实验相称的实验设计、执行和分析 该领域的熟练程度。该资源提供的服务不是布法罗其他任何设施所提供的 地区和费用在全国名列前茅。FICSR的主要目标是保持步伐或 理想情况下，领先于CCSG用户群的细胞学需求。因此，资源领导层将继续密切合作 与CCSG成员进行互动以确定他们的需求，并将监测新的 技术和分析工具。

PROJECT SUMMARY / ABSTRACT The mission of the Flow and Image Cytometry Shared Resource (FICSR) is to provide contemporary advanced flow cytometric analysis and sorting services, as well as imaging services at cellular and subcellular levels of resolution in a cost-effective, user-friendly and scientifically rigorous environment. A major strategic goal of the resource is to provide custom methods development services in a variety of labor-intensive, highly-specialized techniques. Development of methodology is often empirical in nature and cannot be easily obtained from outside sources. During the reporting period, FICSR has acquired new instrumentation, increased capacity, established new services, expanded educational programs, and engaged the Shared Resource Advisory Committee to develop a strategic plan that will enable us to continue to meet the rapidly evolving research needs of CCSG members. FICSR served a total of 134 Roswell users, of which 111 (93%) were CCSG members. There are three types of research investigators who use the FICSR. Level 1 users are those who are proficient in the techniques and know the instruments’ capabilities. For these investigators, the majority of our user base, the resource provides oversight and consultation. Level 2 users depend exclusively on the expertise of the resource staff to provide sample preparation, data acquisition, data analysis and its interpretation. Level 3 users include investigators who are new to flow and/or image cytometry or who are just beginning to consider new applications of this technology to address their research questions. Here the resource staff may perform pilot experiments to determine what would be a useful approach. The Specific Aims of the FICSR are: 1) To provide Roswell Park CCSG members access to cost-effective flow and image cytometry technology which is maintained according to a quality assurance program that follows regulatory agency guidelines and which meets the functional demands of their research programs; 2) To educate Roswell Park CCSG members on new developments in flow and image cytometry technology, their application and implementation pertaining to their specific research areas; 3) To enhance inter- and intra-programmatic collaborations by providing an educational and user-friendly environment in which FICSR personnel assist users with experimental design, execution, and analysis of their experiments commensurate with the user’s level of proficiency in the field. The resource offers services not provided by any other facility in the Buffalo region and the fees are among the lowest in the nation. The main objective of the FICSR is to stay on pace or ideally ahead of the cytometry needs of the CCSG user base. Thus, the resource leadership will continue close interactions with CCSG members to determine their needs and will also monitor the emergence of new technologies and analytic tools.