A novel Lyme disease vaccine

项目摘要 一种新型莱姆病疫苗 获得性抗肩突硬蜱，俗称“蜱抗性”， 可以防止莱姆病病原体伯氏疏螺旋体的传播。我们有 表明在豚鼠（1）反复暴露于I. 肩胛肌（2）免疫I.肩胛肌唾液，或（3）用脂质免疫 含有编码19种唾液蛋白（19ISP）的mRNA的纳米颗粒。此外，本发明还提供了一种方法， 唾液蛋白14（Salp14）是19ISP的一个组成部分，可使蜱产生部分抗性。 本研究的目的是鉴定I.肩突 引发蜱抗性并证明用这些疫苗接种唾液 蛋白质可以预防B。伯氏感染我们将阐述 Salp14和其他唾液蛋白在蜱抗性中决定宿主反应 与蜱抵抗力有关，并检查是否用唾液接种疫苗 编码每种唾液蛋白的蛋白质或mRNA可以预防B。伯氏感染 这些研究将带来新的知识，以帮助开发新的疫苗 这可能会预防莱姆病，也可能会预防其他蜱传感染。

PROJECT SUMMARY A novel vaccine for Lyme disease Acquired resistance to Ixodes scapularis, commonly known as “tick resistance”, can prevent transmission of Borrelia burgdorferi, the Lyme disease agent. We have shown that tick resistance can be generated in guinea pigs (1) repeatedly exposed to I. scapularis, (2) immunized with I. scapularis saliva, or (3) immunized with a lipid nanoparticle containing mRNAs encoding 19 saliva proteins (19ISP). In addition, salivary protein 14 (Salp14), a component of 19ISP, elicits partial tick resistance. The goal of this proposal is to identify the specific proteins in I. scapularis saliva that elicit tick resistance and demonstrate that vaccination with these proteins can prevent B. burgdorferi infection. We will delineate the importance of Salp14 and additional saliva proteins in tick resistance, determine the host responses that are associated with tick resistance, and examine whether vaccination with saliva proteins or mRNAs encoding each saliva protein can prevent B. burgdorferi infection. These studies will lead to new knowledge to aid in the development of a novel vaccine which may prevent Lyme disease and, possibly, other tick-borne infections.