Core A: Animal Models Core

动物模型核心集中育种，发展和维护非常有用，但 复杂的小鼠和斑马鱼模型，通过为每个集中饲养的品系提供稳定的环境， 提高效率、降低成本和防止表型变异。此核心利用特殊的 无病原体（SPF）和无菌小鼠和斑马鱼设施和专家人员提供高质量的 为所有3个当前和拟议项目提供资源和服务。 核心A人员与项目研究人员密切合作，提供SPF和gnotobiotic小鼠， 斑马鱼模型，这是必不可少的，以实现目前的每个项目的目标。该核心提供 优先创建新的SPF小鼠品系和衍生无菌（GF）品系， 项目1、2、3和4的成功。此外，这一核心创造了高度创新的斑马鱼模型， 促进项目3和项目4的活动。通过提供技术经验和中央住房， 保持GF野生型，突变型和双突变型小鼠品系，核心A允许研究人员有效地 进行创新，机械实验远远超出了传统的R01支持的资源， 调查人员和扩大我们国家的gnotobiotic资源的能力。这些GF老鼠和斑马鱼 允许项目研究人员操纵复杂的肠道微生物生态学，以研究 腔微生物对用确定的微生物群定殖的无菌动物的免疫和上皮反应的影响。 动物模型核心的具体目标是为P01项目提供： 1.集中饲养无特定病原菌小鼠和斑马鱼， 繁殖单位的冗余，减少开支，通过维持中央 饲养设施，并通过促进多个研究者共享动物来减少浪费; 2.创建新的小鼠和斑马鱼模型，包括进口现有的突变品系进行集中育种， 通过现有突变体、转基因突变体和 荧光蛋白报告基因系，并产生新的转基因和突变体系; 3.现有和新的小鼠品系的无菌衍生，可用于评估 复杂的常驻微生物群个体微生物物种或微生物聚生体对肠道炎症的影响， 粘膜内稳态 此核心支持更新申请中的项目1、2、3和4。

The Animal Models Core centralizes breeding, development and maintenance of extremely useful, but complex mouse and zebrafish models by providing a stable environment for each centrally housed line to increase efficiency, decrease costs and prevent phenotype variation. This Core leverages exceptional specific pathogen free (SPF) and gnotobiotic mice and zebrafish facilities and expert personnel to provide high-quality resources and services to all 3 current and proposed Projects. Core A personnel work closely with Project investigators to provide SPF and gnotobiotic mouse and zebrafish models that are essential to accomplish goals of each of the current projects. This core provides prioritized creation of novel SPF mouse strains and derivation of germ-free (GF) lines that will support the success of Projects 1, 2, 3 and 4. In addition, this Core has created highly innovative zebrafish models to facilitate activities in Projects 3 and 4. By providing technical experience and central housing to create and maintain GF wild type, mutant and double mutant mouse strains, Core A allows investigators to efficiently perform innovative, mechanistic experiments far beyond the resources of traditional R01-supported investigators and extends the capabilities of our national gnotobiotic resource. These GF mice and zebrafish allows Project investigators to manipulate the complex gut microbial ecology to study the mechanistic role of luminal microbes on immune and epithelial responses in gnotobiotic animals colonized with defined microbiota. The Specific Aims of the Animal Models Core are to provide P01 Projects with: 1.Centralized breeding of specific pathogen free and germ-free mice and zebrafish to eliminate redundancy in breeding units, diminish expenses, decrease environmental variation by maintaining central housing facilities and minimize waste by promoting shared utilization of animals by multiple investigators; 2.Create new mouse and zebrafish models including importing existing mutant strains for central breeding, creating new strains, including cell-specific mutants by crossbreeding of existing mutant, transgenic and fluorescent protein reporter lines, and generating new transgenic and mutant lines; 3.Germ-free derivation of existing and new mouse strains that can be used to evaluate the impact of complex resident microbiota individual microbial species or microbial consortia on intestinal inflammation and mucosal homeostasis. This Core supports Projects 1, 2, 3 and 4 in the renewal application.