Genomic Sciences Core

基因组科学核心（GSC）的目标是为老龄化和老年科学的研究人员提供 这个国家能够获得针对生物学的最先进的基因组、表观基因组和转录组分析 老龄化研究问题，而不是通常由机构核心提供。具体而言，GSC将 提供线粒体基因组分析，DNA修饰（甲基化/羟甲基化）， 和单细胞转录组学。与老年信息学核心协调，这些服务将 从数据生成到分析和解释，由制造商提交的样品。在上一个周期，我们的 表观基因组和线粒体基因组服务得到广泛使用，并将继续提供。新服务 将整合全基因组表观基因组分析、甲基化与羟甲基化的区分以及 一套单细胞RNA-Seq服务。将服务重点放在这些领域的理由是： 线粒体DNA突变/缺失和拷贝数变化是一个中心的机制假设， 线粒体功能障碍2)表观遗传机制是一种保守的，潜在的致病机制， 老化的因素。越来越多的证据表明，不仅表观基因组会随着衰老而变化， 衰老干预可以防止表观基因组发生与年龄相关的变化。3)现代分子生物学开启了 了解细胞异质性的新途径当前老年科学研究的核心研究领域 (e.g.,细胞衰老）。单细胞转录组学方法超越了整个组织的批量RNA-Seq， 单个细胞类型和亚型。GSC服务设计的核心是生物信息学工作流程， 研究人员从样本到全面分析的数据。GSC将有三个目标：1）定量精确 DNA修饰分析，从全基因组到基因特异性，使用先进的表观遗传学 技术，2）测量线粒体基因组异质性和拷贝数，用于综合变体 分析和绝对定量，和3）用于分析细胞异质性的单细胞转录组学。GSC 服务的设计，使老龄化研究调查人员可以利用具体的分析或整体 工作流程满足他们的实验需求，几乎所有的服务都可以在存储的核酸上执行。 酸/细胞。基因组科学核心开发了一套与衰老研究高度相关的工具， 研究人员无法在自己的实验室或通过机构核心设施广泛获得， 仪器和专业知识的条款，满足在该领域的资源提供者的需要。而且 与GeroInformatics Core的互动提供了一个工作流程，不仅可以让老年科学研究人员从 全国各地都有GSC生成的数据，但这些数据被分析成一种可用的形式。

The goal of the Genomic Sciences Core (GSC) is to provide researchers in aging and geroscience across the country with access to state-of-the-art genomic, epigenomic, and transcriptomic analyses targeted to biology of aging research questions and that are not typically provided by institutional cores. Specifically, the GSC will offer services in the analysis of mitochondrial genomes, DNA modifications (methylation/hydroxymethylation), and single-cell transcriptomics. In coordination with the GeroInformatics Core, these services will take investigator-submitted samples from data generation to analysis and interpretation. In the previous cycle, our epigenomic and mitochondrial genomic services were widely used and will continue to be offered. New services will integrate whole genome epigenomic analyses, differentiation of methylation from hydroxymethylation and a suite of single cell RNA-Seq services. The rationale for focusing services in these areas are: 1) Accumulation of mitochondrial DNA mutations/deletions and copy number changes are a central mechanistic hypothesis in mitochondrial dysfunction with aging. 2) Epigenetic mechanisms are a conserved, and potentially causative, factor in aging. Growing evidence demonstrates that not only does the epigenome change with aging, but anti- aging interventions can prevent age-related changes to the epigenome. 3) Modern molecular biology has opened a new door to understand cellular heterogeneity a research area at the heart of current geroscience research (e.g., cellular senescence). Single-cell transcriptomic approaches move past bulk RNA-Seq of whole tissues to individual cell types and sub-types. Central to the design of the GSC services are bioinformatic workflows to take investigators from sample to fully analyzed data. The GSC will have three aims: 1) Perform quantitatively precise DNA modification analyses, ranging from genome-wide to gene-specific using advanced epigenetic technologies, 2) Measure mitochondrial genome heteroplasmy and copy number for comprehensive variant analysis and absolute quantitation, and 3) Single-cell transcriptomics for analysis of cellular heterogeneity. GSC services are designed such that aging research investigators can take advantage of specific analyses or whole workflows as meets their experimental needs, and almost all services can be performed on stored nucleic acids/cells. The Genomic Sciences Core has developed a set of tools highly relevant to aging research that are not widely available to investigators, either in their own laboratories or through institutional core facilities, both in terms of instrumentation and expertise, meeting a need for a resource provider in the field. Furthermore, the interaction with the GeroInformatics Core provides a workflow where not only can geroscience investigators from across the country have data generated by the GSC but it is analyzed into an investigator-usable form.