Genomics Shared Resource

基因组学共享资源 项目摘要/摘要 基因组学共享资源(GSR)提供加州大学戴维斯综合癌症中心(UCDCCC) 在基因组学和生物信息学的广泛领域拥有全面服务和专业知识的研究人员，以及 获取最当代的基因组应用程序和最先进的仪器，用于下一代 测序(NGS)和纳米串计数器分析。这些技术结合在一起，使调查人员能够 完成几乎所有类型的基因组应用，包括突变筛选(完整外显子组 测序；WES)，转录组分析(RNA测序)，表观基因组学(ChIP-Seq，Hi-C)，元基因组学， 以及单细胞测序。GSR还为翻译基因组学提供了广泛的服务 具有用于分析来自广泛来源的临床样本的优化方案的研究(例如， FFPE组织、全血、血清、唾液、患者来源的异种移植)和极其有限的数量。 此外，由调查员推动的项目还得到了定制方案和 分析循环肿瘤DNA、胞外体的试剂，包括靶向NGS板和方法 MiRNA和病毒基因组。为了帮助从基因组学实验中获得丰硕的成果，GSR 提供广泛的数据分析和综合生物信息学支持。GSR进一步促进了这些 通过为实验设计、项目后勤和管理提供咨询，以及 支持编写手稿和赠款提案。教育是一个额外的重要因素 是GSR的组成部分，通过实验室培训、讲习班和研讨会提供。监督 GSR的管理由总监John McFherson博士提供，他在以下方面具有深厚的专业知识 DNA测序和癌症基因组学通过人类基因组计划和职业生涯跨越三年 基因组中心；以及联席主任Clifford Tepper博士，他在基因组中心拥有20年的 广泛的基因组学和生物信息学应用的应用和发展。总而言之， GSR为UCDCCC提供了完整的一体化和功能性的分子图谱解决方案。具体的 GSR的目标是：1)向UCDCCC提供基因组学领域的服务、专业知识和技术 调查人员。2)进行生物信息学分析，以提供可解释的基因组学结果 学习。3)通过教育、指导和支持，促进UCDCCC的基因组研究工作 用于准备手稿和赠款提案。4)便利获得UCDCCC的其他“经济服务”。

GENOMICS SHARED RESOURCE (GSR) PROJECT SUMMARY/ABSTRACT The Genomics Shared Resource (GSR) provides UC Davis Comprehensive Cancer Center (UCDCCC) researchers with comprehensive services and expertise in the broad area of genomics and bioinformatics, and access to the most contemporary genomic applications and state-of-the-art instrumentation for next-generation sequencing (NGS) and NanoString nCounter analyses. Together, these technologies enable investigators to accomplish virtually every type of genomics application including mutation screening (whole-exome sequencing; WES), transcriptome profiling (RNA-Sequencing), epigenomics (ChIP-Seq, Hi-C), metagenomics, and single-cell sequencing. The GSR also provides a wide range of services for translational genomics research with optimized protocols for the analysis of clinical samples from a broad range of sources (e.g., FFPE tissue, whole blood, serum, saliva, patient-derived xenografts) and of extremely limited amounts. Moreover, investigator-driven projects have been supported by the development of customized protocols and reagents, including targeted NGS panels and methods for the analysis of circulating tumor DNA, exosomal miRNA, and viral genomes. To help achieve productive outcomes from genomics experiments, the GSR provides extensive data analysis and integrative bioinformatics support. The GSR further facilitates these projects by providing consultation for experimental design, project logistics and management, as well as support for the preparation of manuscripts and grant proposals. Education is an additional important component of the GSR and is provided through laboratory-based training, workshops, and seminars. Oversight and management of the GSR is provided by the Director, John McPherson, PhD, who has deep expertise in DNA sequencing and cancer genomics through the Human Genome Project and a career that spans three Genome Centers; and the co-Director, Clifford Tepper, PhD, who has 20 years of experience in the application and development of a wide variety of genomics and bioinformatics applications. Taken together, the GSR provides a complete integrative and functional molecular profiling solution to the UCDCCC. The Specific Aims for the GSR are to: 1) Provide services, expertise, and technologies in the area of genomics to UCDCCC investigators. 2) Perform bioinformatics analysis in order to provide interpretable results from genomics studies. 3) Facilitate the genomics research efforts of the UCDCCC through education, mentoring and support for preparation of manuscripts and grant proposals. 4) Facilitate access to other `omic services at UCDCCC.