Charge Transfer Study of DNA/MoS2 interface

项目摘要 串联DNA重复序列的扩增导致40多种遗传转移性疾病， 每年影响四百万人目前最先进的遗传诊断技术 长度突变的测试有其自身的局限性，例如蛋白质纳米孔的堵塞，需要 标记步骤、频繁的假阳性/阴性结果或短碱基对读取长度。克服 限制阻碍了当前的生物医学科学，迫切需要开发新的平台 建立在彻底的基础科学之上。该地区的建议主要涉及本科研究人员 研究了与二硫化钼表面连接的串联DNA重复序列的内在特征， 用于将来重复突变的无标记传感平台。 PI假设DNA重复序列可以产生依赖于序列和长度的电荷 由于核碱基对二维材料的不同亲和力，即， 二硫化钼（MoS 2）。这是一个关键的信息，需要通过一个 严谨的研究。基于令人鼓舞的初步结果，本项目旨在从根本上 电化学和表面探针显微镜研究DNA/MoS 2界面 技术.在具体的目标中，PI计划（1）研究序列依赖性电荷传输， TNR/2D纳米材料界面，以及（2）研究它们相对于序列长度的行为。的 结果也将与浓度和构象对电荷转移的影响进行比较 性格 该地区的建议将使本科研究人员接触到高质量的研究， 表面化学和材料科学，在生物医学研究中具有最终应用， 改善公共卫生。完成后，我们将能够更好地应用DNA/2D材料， 对重复突变进行选择性和灵敏的检测，这将最终改善数百万人的生活 个人。

Project Summary Expansion of tandem DNA repeats cause more than forty genetically transferrable disorders, which affect 4 million people every year. Current state-of-the-art diagnostic technologies for genetic testing for length mutations have their own limitations such as clogging of protein nanopores, requiring labelling steps, frequent false positive/negative results, or short basepair read length. To overcome the limitations that hamper the current biomedical science, there is a critical need to develop new platforms founded on thorough basic science. This AREA proposal involving mainly undergraduate researchers investigates intrinsic character of tandem DNA repeats interfaced with MoS2 surfaces that may manifest into label-free sensing platform for repeat mutations in future. The PI hypothesizes that DNA repeats can produce sequence- and length-dependent charge transfer signals due to the differential affinity of nucleobases for two-dimensional materials, i.e. molybdenum disulfide (MoS2). This is the critical piece of information needed to confirm through a rigorous study. Based on encouraging preliminary results, current project is designed to fundamentally investigate DNA/MoS2 interfaces in detail by electrochemical and surface probe microscopy techniques. In the specific aims, the PI plans to (1) investigate sequence-dependent charge transport at TNR/2D nanomaterials interface, and (2) investigate their behavior with respect to sequence length. The results will also be compared with concentration and conformations effects on the charge transfer character. This AREA proposal will expose the undergraduate researchers to high-quality research in surface chemistry and materials science, which has ultimate application in biomedical research to improve public health. Upon completion, we will be in better position to apply DNA/2D materials for selective and sensitive detection of repeat mutations, which will ultimately improve the lives of millions of individuals.