Light Microscopy Core

抽象光学显微镜共享资源 光学显微镜共享资源(LM)为固定设备提供最先进的光学显微镜技术 以及活细胞和组织，以及科学和技术专长，以协助实验设计和 最佳图像收集。通过LM共享资源，您可以经济高效地访问超过800万美元 显微成像资源，包括共焦和常规荧光仪器、多光子、 向杜克癌症研究所(DCI)的所有成员提供LightSheet和超分辨率。三个全职的专职人员 博士级别的工作人员为所有显微镜系统提供培训和全面的技术支持。DCI成员 优先获得设备、培训课程和工作人员援助。实践个人化 在其他用户之前，应要求向DCI成员提供分析方面的培训和协助。LM是 每年由用户费用和DCI CCSG、杜克大学学院 医学和杜克大学教务长办公室。全标准光学切片和荧光 提供的显微技术包括共聚焦、共振扫描共聚焦、旋转圆盘、活细胞 几种方式的成像，长期孵化时间推移，多光子，光激活/光转化 和光动(FRAP，翻转)能力，此外，新技术-光版和超分辨率。 订座通过全校软件系统以先到先得的方式在网上进行。 有一些限制，以促进有效使用。具有一定复制功能的大范围设备 为用户的需求提供了良好的能力。Lm的首要任务是满足DCI的光学显微镜需求 成员和所有杜克大学的研究人员在设备和图像分析方面提供支持和帮助，以便 提高工作效率。同时，我们通过演示和测试新的和不同的技术来保持最新 并努力实施被证明有价值的新技术。 2018年，LM为233名调查人员提供了服务，其中40%是DCI成员，占55% 来自所有8个DCI研究项目的总使用量。DCI成员使用这一共享资源有助于 在项目期间发表了261篇文章，其中71篇发表在影响较大的期刊上(IF&>9)，展示了其价值 由资源提供的服务的。

ABSTRACT - LIGHT MICROSCOPY SHARED RESOURCE The Light Microscopy Shared Resource (LM) provides state-of-the-art light microscopy technology for fixed and live cells and tissues, along with scientific and technical expertise to assist in experimental design and optimal image collection. The LM shared resource offers affordable and efficient access to over $8 million in microscopic imaging resources, including confocal and conventional fluorescence instruments, multi-photon, lightsheet and super-resolution to all members of the Duke Cancer Institute (DCI). Three dedicated, full-time PhD-level staff members provide training and full technical support for all the microscopy systems. DCI members receive special priority access to equipment, training sessions and staff assistance. Hands-on individualized training and assistance with analysis is provided upon request to DCI members in advance of other users. LM is supported annually with charge backs from user fees and by the DCI CCSG, the Duke University School of Medicine and the Duke University Office of the Provost. All standard optical sectioning and fluorescence microscopy technologies are offered including confocal, resonant scanning confocal, spinning disk, live-cell imaging with several modalities, long-term incubated time-lapse, multi-photon, photoactivation/photoconversion and photokinetic (FRAP, FLIP) capabilities, and in addition, new technologies – lightsheet and super-resolution. Reservations are executed online through the university-wide software system on a first-come, first-served basis with some limits to promote efficient use. The large range of equipment with some replication of capabilities provides good capacity for users’ needs. LM’s continued priority is to serve the light microscopy needs of DCI members and all Duke researchers with support and assistance on equipment and image analysis in order to generate productivity. At the same time we keep current by demo-ing and testing new and different technologies and strive to implement new technologies that prove valuable. In 2018, LM provided services to 233 investigators, 40% of whom were DCI members, accounting for 55% of total usage, from all 8 DCI Research Programs. Use of this shared resource by DCI Members contributed to 261 publications over the project period, 71 of which were in high impact journals (IF>9), demonstrating the value of services offered by the resource.