Expanding regional capacity for single molecule sequencing through the purchase of the Sequel IIe sequencing system

项目总结 路易斯维尔大学测序技术中心(STC)正在请求支持，以获得太平洋 生物科学续集IIe测序系统。所请求的系统将是年中的第二个续集IIe系统 该中心应不同STC用户群的需求，包括区域、国家和国际用户群 外部资助的调查人员已经远远超出了我们最初单一工具的能力。自推出以来 2021年，STC协助规划、准备和执行了许多利用权力的项目 使用标准高保真(HiFi)的项目的长读、单分子、实时(SMRT)测序 工作流程，以及涉及开发定制的、有针对性的创新方法以阐明复杂情况的工作流程 具有阶段性、高度精确度的长阅读的基因组位点或群落。与其他测序系统不同 市场上，Sequel IIe系统具有长读能力(150个BP-100 kb)和最高的 任何NGS和单分子测序仪的准确性(从Q30，99.9%到 Q60，99.9999%)，这两个因素对于满足我们的主要、次要和 服务用户。STC已经建立得很好，并且已经具备了以下基础设施和运营能力 实施这个要求的第二续集IIe系统，这是绝对必要的，以满足我们的需求 不断增长的用户基础，主要用户研究项目，以及使用POWER的进一步基因组学方法开发 SMRT测序技术。路易斯维尔大学，特别是研究和创新办公室， 致力于通过对STC的财务和基础设施投资来支持所要求的系统。 从产生新的参考基因组到有针对性地描述病毒库和免疫位点， 和至关重要的SARS-CoV-2病原体监测遍及肯塔基州，STC 工厂已经并将利用其在SMRT测序和拟议的新Sequel IIe系统方面的专业知识来工作 与当地和国家专家密切合作，促进各种领域的尖端基因组学项目 研究领域，主要目标是促进和激励研究和创新，以支持增长 路易斯维尔大学的研究项目。

PROJECT SUMMARY The University of Louisville’s Sequencing Technology Center (STC) is requesting support to acquire a Pacific Biosciences Sequel IIe sequencing system. The requested system would be the second Sequel IIe system in the Center, as demand from the diverse STC user base, comprised of regional, national, and international extramurally funded investigators has far exceeded the capacity of our initial, single instrument. Since launching in 2021, the STC has assisted in the planning, preparation, and execution of many projects leveraging the power of long read, single molecule, real-time (SMRT) sequencing for projects that use both standard high fidelity (HiFi) workflows, and those that involve development of custom, innovative targeted methods to elucidate complex genomic loci or communities with phased, highly accurate long reads. Unlike other sequencing systems on the market, the Sequel IIe system has the combination of long read capabilities (150 bp – 100 kb) and the highest accuracy available from any NGS- and single molecule-sequencing instrument (ranging from Q30, 99.9% - to Q60, 99.9999%, across our projects), both of which are critical for meeting the needs of our Major, Minor, and service users. The STC is well established and already has the infrastructure and operational capabilities to implement this requested second Sequel IIe system, which is absolutely necessary for meeting the needs of our growing user base, Major User research projects, and further genomics methods development using the power of SMRT sequencing technology. The University of Louisville, particularly the Office of Research and Innovation, is dedicated to supporting the requested system through financial and infrastructure investment into the STC. From the generation of new reference genomes to targeted characterization of viral reservoirs and immune loci, and critically important SARS-CoV-2 pathogen surveillance across the Commonwealth of Kentucky, the STC facility has and will use its expertise with SMRT sequencing and the proposed new Sequel IIe system to work closely with local and national experts to facilitate cutting-edge genomics projects across a wide variety of research areas with the major goal of promoting and inspiring research and innovation in support of the growth of the research enterprise at the University of Louisville.