Neuroanatomy/Histology/Brain Injection Core

摘要 神经解剖学/组织学/脑注射核心（核心C）将促进神经解剖学和组织学 PPG成员的研究。PPG中描述的所有3个项目的研究者将定期使用它。博士 Laurent Gautron将协调这些服务和资源，并将定期提供人员培训 关于设备的使用。核心C也将是表征和验证的重要组成部分 3个项目提出的基因特异性缺失小鼠模型。核心C配备了设备， 专业知识，提供以下服务： Core C将提供以下服务： 常规组织学检查 免疫标记 RNA原位杂交 立体定向手术显微镜 脑显微解剖 RNA分离和qPCR分析。 Cores C的技术专长将为项目调查人员提供多种好处： 集中的核心服务更具成本效益，并可防止试剂重复使用。 项目调查员。 统一的标准和协议将确保所有项目的结果可以直接进行比较 为项目调查人员不经常执行的方案提供技术专业知识

ABSTRACT The Neuroanatomy/Histology/Brain Injection Core (Core C) will facilitate neuroanatomical and histological studies for PPG Members. It will be used regularly by investigators from all 3 projects described in the PPG. Dr. Laurent Gautron will coordinate these services and resources, and will provide periodic training of personnel on the use of the equipment. Core C will also be an essential component of the characterization and validation of gene specific deletion mouse models proposed by all 3 projects. Core C is equipped with the equipment and expertise to provide the following services: Core C will provide the following services:  Routine histology  Immunolabeling  RNA in situ hybridization  Stereotaxic surgeriesMicroscopy  Brain microdissection  RNA isolation and qPCR analysis. The technical expertise in Cores C will provide multiple benefits to project investigators:  Centralized Core services are more cost effective and prevent the duplication of reagents across Program investigators.  Uniform standards and protocols will ensure that results from all Projects can directly be compared  Provides technical expertise for protocols that are not routinely performed by Program investigators