Neuroanatomy/Histology/Brain Injection Core

神经解剖学/组织学/脑注射核心

• 批准号: 10018901
• 负责人: Laurent Gautron
• 金额: $ 23.91万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-20 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10018901
• 关键词: Biological Assay; Brain; Cannulations; Cerebrospinal Fluid; Collection; Energy Metabolism; Ensure; Equipment; Exercise; Fluorescent in Situ Hybridization; Frozen Sections; Genes; Histologic; Histology; Human Resources; Immunohistochemistry; In Situ Hybridization; Injections; Light Microscope; Link; Microdissection; Neuroanatomy; Neurons; Operative Surgical Procedures; Paraffin; Periodicity; Population; Protocols documentation; RNA; Reagent; Research; Research Personnel; Resources; Role; Services; Slide; Technical Expertise; Tissues; Training; Validation; cost effective; energy balance; exercise training; experimental study; fluorescence microscope; histological stains; histological studies; laser capture microdissection; light microscopy; member; metabolic profile; mouse model; prevent; programs

ABSTRACT The Neuroanatomy/Histology/Brain Injection Core (Core C) will facilitate neuroanatomical and histological studies for PPG Members. It will be used regularly by investigators from all 3 projects described in the PPG. Dr. Laurent Gautron will coordinate these services and resources, and will provide periodic training of personnel on the use of the equipment. Core C will also be an essential component of the characterization and validation of gene specific deletion mouse models proposed by all 3 projects. Core C is equipped with the equipment and expertise to provide the following services: Core C will provide the following services:  Routine histology  Immunolabeling  RNA in situ hybridization  Stereotaxic surgeriesMicroscopy  Brain microdissection  RNA isolation and qPCR analysis. The technical expertise in Cores C will provide multiple benefits to project investigators:  Centralized Core services are more cost effective and prevent the duplication of reagents across Program investigators.  Uniform standards and protocols will ensure that results from all Projects can directly be compared  Provides technical expertise for protocols that are not routinely performed by Program investigators

摘要