Cell Imaging and Histology

摘要-细胞成像和组织学共享资源 由癌症中心管理的细胞成像和组织学共享资源的使命是 为中心研究人员提供最先进的成像和组织学服务。核心提供了 仪器，专业知识，培训和服务，以实现先进的细胞成像和数据分析。细胞 成像设施的技术包括最近获得的混合共焦/超分辨率尼康A1显微镜 系统配备活细胞成像，以及其他三个共聚焦显微镜系统-蔡司LSM 710 配备多光子激光器、Olympus FloView 1000和Yokogawa的NLO激光点扫描系统 旋转圆盘共聚焦显微镜此外，该设施还有其他六台荧光显微镜， 广泛的成像技术，如FRET，TIRF，活细胞和延时成像。大多数先进的 SBP的显微镜位于设施内。该设施的计算机工作站提供了访问图像 分析软件包，包括Image-Pro plus、MetaMorph、Volocity和Imaris 8.0，以及Nikon 尼康超分辨率数据离线重建软件。组织学设施服务包括 组织的制备和冷冻切片; H&E自动染色（Leica XLf），和免疫组织化学， 免疫荧光和原位杂交（Leica Bond RX）。该机构的Aperio载玻片扫描仪（AT 2和FL ScanScopes，Leica）提供全IHC或荧光染色载玻片的自动高分辨率扫描， 数据记录、分析、存储和传播。该设施还提供援助， 人体组织，包括协助IRB和MTA问题。邻近的细胞成像和组织学 设施提供用户培训或全方位服务。经过培训的显微镜用户可以全天候使用设备， 通过在线iLab日历预订每台仪器。Core的使用非常广泛， 在过去的5年里，核心为47个癌症中心成员实验室的研究做出了贡献，这些实验室代表了所有 三个中心的方案，并协助项目产生了190多个癌症相关的出版物。

ABSTRACT – CELL IMAGING AND HISTOLOGY SHARED RESOURCE The mission of the Cell Imaging and Histology Shared Resource, which is managed by the Cancer Center, is to provide state-of-the-art imaging and histological services to Center researchers. The Core offers the instrumentation, expertise, training, and service to enable advanced cellular imaging and data analysis. The Cell Imaging facility's technology includes a recently acquired hybrid confocal/super-resolution Nikon A1 microscope system equipped for live-cell imaging, as well as three other confocal microscope systems—a Zeiss LSM 710 NLO laser point scanning system equipped with a multiphoton laser, An Olympus FloView 1000, and a Yokogawa spinning disk confocal microscope. In addition, the facility has six other fluorescence microscopes supporting a wide range of imaging technologies such as FRET, TIRF, live cell and time-lapse imaging. Most of the advanced microscopes at SBP are located within the facility. The facility's computer workstations provide access to image analysis software packages including Image-Pro plus, MetaMorph, Volocity, and Imaris 8.0, as well as Nikon software for off-line reconstruction of Nikon Super-Resolution data. The Histology facility services include preparation and cryosectioning of tissues; automated staining for H&E (Leica XLf), and immunohistochemistry, immunofluorescence, and in situ hybridization (Leica Bond RX). The facility's Aperio slide scanners (AT2 and FL ScanScopes, Leica) provide automated high-resolution scanning of whole IHC or fluorescence stained slides for data documentation, analysis, storage, and dissemination. The facility also provides assistance in obtaining human tissues, including assistance with IRB and MTA issues. Both the adjacent Cell Imaging and Histology facilities provide user training or full-service. Trained microscope users have 24/7 access to the equipment, with reservations for each instrument made through an on-line iLab calendar. The Core is very broadly used—in the past 5 years, the Core has contributed to research in 47 Cancer Center member laboratories representing all three Center programs and assisted in projects resulting in more than 190 cancer-relevant publications.