A System for the Large‐scale Production of Membrane Proteins

膜蛋白在细胞过程中起着基础和关键的作用。它们直接链接到 人类疾病及其在信号转导中的关键作用使许多膜蛋白 化疗干预的首要目标是使用超过50%的旨在调节肿瘤生长的药物 这类蛋白质的活性。尽管在人类健康中发挥着核心作用，但生化、功能和 膜蛋白的结构性质仍然知之甚少。这在一定程度上是由于 分离生化所需的足够数量和纯度的膜蛋白所面临的挑战 人物刻画。利用异源蛋白表达系统合成蛋白质的传统方法 重组膜蛋白已经取得了一定的成功。没有一种制度是普遍存在的 生产重组膜蛋白的过程仍然是一个反复试验的过程。至 在膜蛋白研究和新的治疗选择的开发中解决这一瓶颈， 我们提出了利用单细胞绿藻衣藻的类囊体膜系统作为 这是一种更适合重组膜蛋白生产的平台。建议的研究目标 通过构建一组专门的载体来演示使用这种系统的可行性 膜蛋白在类囊体膜上的表达及系统的实用性 与医学相关的膜蛋白。

Membrane proteins play a fundamental and critical role in cellular processes. Their direct link to human disease as well as their key role in signal transduction, has made many membrane proteins the foremost targets for chemotherapeutic intervention with more than 50% of all drugs designed to modulate the activity of this class of proteins. Despite this central role in human health, the biochemical, functional and structural properties of membrane proteins remain poorly understood. This is partially due to the inherent challenges of isolating membrane proteins in sufficient quantities and levels of purity needed for biochemical characterization. The traditional approach of using heterologous protein expression systems to synthesize recombinant membrane proteins has been marginally successful. No one system has emerged as universally applicable and the process of producing recombinant membrane proteins remains one of trial and error. To address this bottleneck in the study of membrane proteins and in the development of new treatment options, we have proposed the use of the thylakoid membrane system of the unicellular green algae Chlamydomonas as a more suitable platform for the production of recombinant membrane proteins. The proposed research aims to demonstrate the feasibility of using such a system by building a set of specialized vectors that direct the expression of membrane proteins to the thylakoid membrane and to test the utility of the system using two medically relevant membrane proteins.