One Test, One Kit for Identifying Active Hepatitis C Virus (HCV) Infection at Home

Zymeron的目标是开发用户友好的技术和设备，以实现丙型肝炎病毒(HCV)检测 回家。不需要特殊培训，任何人都可以从手指抽血中制备丙型肝炎病毒RNA样本，用于 随后的等温放大和纸基纳米增敏检测只需如下操作 简单的说明。Zymeron将为实现这一目标而开发的两项创新是：(I)易于实现 使用血液处理设备，允许最终用户从指尖采集血液并分离血浆 自动；和(Ii)一种新型的纸质测试盒，集成了多步核酸扩增 在单个设备中进行测试(NAAT)。基于成功使用这些经过验证的内部开发的创新 对于护理点(POC)测试，Zymeron将进一步推进这些技术，使其适用于 家庭检测丙型肝炎病毒，为任何潜在的最终用户提供最简单的常规操作，以始终运行试剂盒 他们自己。对于一个长期目标来说，这项提案中发明的创新不仅将具体受益 用于丙型肝炎病毒筛查，也用于在资源有限的环境中进行其他家庭和POC测试，这些环境依赖于快速 血浆样品的NaT。

Zymeron aims to develop user-friendly technologies and devices to achieve Hepatitis C Virus (HCV) testing at home. Without special training required, anyone can prepare an HCV RNA sample from fingerprick blood for subsequent isothermal amplification and paper-based nanoenhanced sensitive detection just by following simple instructions. The two innovations that Zymeron will develop to achieve such an aim are (i) an easy to use blood processing device that allows an end user to collect blood from fingerprick and separate plasma automatically; and (ii) a novel paper-based test cassette that integrates multistep nucleic acids amplification testing (NAATs) in one single device. Based on the success of using these proven innovations developed inhouse for Point-of-Care (POC) testing, Zymeron will further advance these technologies to be suitable for home testing of HCV, providing the simplest routine operation for any potential end user to run the kit all by themselves. For a long-term goal, the innovation invented in this proposal will not only benefit specifically for HCV screening but also for other at-home and POC tests in resource-limited settings that rely upon rapid NAAT of plasma samples.