Multi-modality optical imaging of single-cell dynamics using supercontinuum light source

项目摘要 本研究的目的是开发一种多模态成像平台， 基于光学计算和荧光显微术的复相位显微术 （FM）.我们将使用成像平台来关联亚细胞动态运动， 细胞内的特性，以研究细胞粘附，这在许多方面起着重要作用， 细胞行为本研究对基础研究、临床前和临床研究具有重要意义， 和技术发展。通过同时成像细胞运动和细胞器特性， CPM-FM使相关生物分子特性的细胞动力学研究成为可能。 CPM-FM对细胞粘附的研究将使我们更好地理解细胞粘附的病理生理学。 不同的疾病，为疾病治疗和早期诊断的新方法打开了大门， 导致开发需要与细胞特异性相互作用的生物材料， 患者和临床医生。本研究还将建立CPM作为成像的可行性 一种允许无创、连续监测细胞活动而无需造影剂的技术 剂.特别地，提供了一种具有宽带宽、高空间分辨率和高分辨率的超连续谱光源， 将获得相干性和大的输出功率并用作成像的光源 系统，实现纳米级位移灵敏度，亚微米级空间分辨率 和丰富的分子信息。的获取 强大的光源，预计将大大提高我们的研究能力。

Project Summary The objective of this study is to develop a multi-modality imaging platform that integrates complex phase microscopy (CPM) based on optical computation and fluorescence microscopy (FM). We will use the imaging platform to correlate the sub-cellular dynamic motion and intracellular properties to study cell adhesion which plays an important role in many aspects of cell behavior. This study is significant for fundamental research, preclinical and clinical study, and technology development. By simultaneously imaging cell motion and organelle properties, CPM-FM enables the investigation of cell dynamics in correlation biomolecular characteristics. CPM-FM investigation of cell adhesion will result in better understanding of pathophysiology of different diseases, open the door to new methods for disease treatment and early diagnosis, lead to the development biomaterials that need specific interaction with cells, and benefit patients and clinicians. This study will also establish the feasibility of CPM as an imaging technology that allows non-invasive, and continuous monitoring of cell activities without contrast agents. Particularly, a supercontinuum light source with broad bandwidth, high spatial coherence and large output power will be acquired and used as the light source for the imaging system, to achieve nanoscale displacement sensitivity, sub-micrometer level spatial resolution and rich molecular information from multiple fluorescence probes. The acquisition of the powerful light source is expected to enhance our research capability significantly.