Circadian regulation of brain and body in larval zebrafish

项目摘要 昼夜节律通过对细胞靶点的不同作用，彻底改变了动物的行为。 身体睡眠-活动周期的中断是影响行为、神经学、 新陈代谢和肌肉生理学。该提案提出了一系列旨在了解 生理节律对身体细胞和系统的影响机制和相互作用。昼夜节律 通过直接作用于目标以调节生理的信号分子在起搏器下游显现。 了解不同的目标是如何被修改，以实现一个星座的生理和行为 节律是时间生物学研究的主要目标。我们研究神经元、肌肉和生物力学 相互作用的幼体斑马鱼，一个听话的昼夜脊椎动物，我们的初步实验表明，这些动物 经历的昼夜节律变化的广度远比以前所知的更加多样化。虽然斑马鱼是一种 不完善的睡眠行为模型，其明确的昼夜节律结合其对生理的易驾驭性， 行为和遗传的方法使其成为了解昼夜节律如何组织的理想系统 并在细胞和器官间相互作用。最初的建议旨在确定不同的昼夜节律对 通过跟踪和建模，神经系统输出和肌肉生理学相当于复杂的行为输出 斑马鱼的行为和昼夜周期中的觉醒。在此，我们请求提供资源， 用于多光子显微镜的支撑件，该多光子显微镜利用不可见光提供活性测量， 促进跨昼夜节律时间的生理学比较，而没有测量的混杂效应 使用可见光的方法。这些实验将提供详细的信息和模型 关于细胞和系统之间昼夜节律效应的相互作用。

Project Summary Circadian rhythms drastically alter animal behavior through diverse actions on cellular targets throughout the body. Disruptions of sleep-activity cycles account for a wide range of diseases affecting behavior, neurology, metabolism, and muscle physiology. This proposal presents a set of studies designed to understand the mechanisms and interactions of circadian effects on cells and systems spanning the body. Circadian rhythms manifest downstream of pacemakers via signaling molecules that act directly on targets to regulate physiology. Understanding how distinct targets are modified to achieve a constellation of physiological and behavioral rhythms is a major goal in chronobiological research. We study how neurons, muscles, and biomechanics interact in larval zebrafish, a tractable diurnal vertebrate, and our preliminary experiments suggest these animals experience a breadth of circadian changes far more diverse than previously known. Although the zebrafish is an imperfect model of sleep behavior, its clear circadian rhythms combined with its tractability for physiological, behavioral, and genetic approaches make it an ideal system for understanding how circadian rhythms organize and interact across cells and organs. The original proposal aims to define how diverse circadian effects on nervous system output and muscle physiology amount to complex behavioral output, by tracking and modeling zebrafish behavior and arousal across the diel cycle. Here we request resources to complement institutional support for a multiphoton microscope, which affords activity measurements using invisible light, thereby facilitating comparisons of physiology across circadian time without the confounding effects of measurement approaches that use visible light. Together these experiments will provide detailed information and models regarding the interaction of circadian effects across cells and systems.