Genetic factors controlling the intensity of social behavior

项目摘要 家长奖(R35GM119844)的这项设备补充的目标是扩展 朝日实验室的多光子成像显微镜，以便活苍蝇大脑中的神经活动可以 在实验者施加复杂的感官刺激和药理试剂的同时进行监测， 光遗传学和热遗传学控制特定的神经元群体，并记录行为。至 要达到父母资助中列出的目标，越来越有必要了解生理学 攻击性控制回路的动力学。计算一种特定社会行为在 以上下文依赖的方式，苍蝇的大脑必须根据 动物的营养和荷尔蒙状况，以及每种动物独特的个体行为史 个人的。受者实验室目前可用的功能成像设置不足以定量地 检测感觉线索的上下文相关表征，因为显微镜的过时设计 约束物镜附近的可用空间。拟购买的设备是 带有电动XY-Stage的龙门架。车架的简化支撑结构将消除 环绕物镜的显微镜的不必要的填充空间部分，而工作台提供 安装空间足以设置多个刺激传递设置、LED、热探头和其他设备 用来进行神经操作。这些特点将极大地促进成功完成 父母补助。

Project Summary The goal of this equipment supplement for the parental award (R35GM119844) is to expand the capability of multiphoton imaging microscopy in the Asahina lab so that neural activities in the brain of a live fly can be monitored while the experimenter applies complex sensory stimuli and pharmacological reagents, optogenetically and thermogenetically manipulates specific neuronal population, and records behavior. To achieve the goals outlined in the parental grant, it is increasingly necessary to understand physiological dynamics of aggression-controlling circuits. To calculate relative cost and benefit of a given social behavior in a context-dependent manner, the fly brain must amplify or diminish relevant sensory information according to the animal’s nutritional and hormonal conditions, as well as the individual behavioral history unique to each individual. The functional imaging setup currently available in the recipient’s lab is inadequate to quantitatively detect context-dependent representation of sensory cues, because the outdated design of the microscopy constrains the space available near the objective lens. The proposed piece of equipment for purchase is a gantry frame with a motorized XY-stage. The simplified support structure of the frame will eliminate unnecessary space-filling parts of the microscopy that surround the objective lens, while the stage provides a mounting space sufficient for setting up multiple stimulus-delivery setups, LED, heat probe, and other devices for neuronal manipulations. These features will greatly facilitate the successful completion of the goals of the parental grant.