Cytoskeletal Function in C. elegans Embryos

项目摘要 Alyssa将使用分子遗传学和高分辨率光学显微镜来进一步推进她的研究。 发现微管是C.线虫卵母细胞皮层， 它们用于覆盖皮层，从而限制膜侵入的程度， 卵母细胞减数分裂和极体排出。通过额外的培训，Alyssa将 获得广泛的分子遗传学新技能（光遗传学，基因替代和 基因组编辑）和显微镜（靠近TIRF、Zeiss Airyscan和Nikon Spinning Disk索拉 显微术）。她还将获得合作，跨学科研究的丰富经验 与我们的成像设备博士亚当弗里斯和高级研究助理在我的实验室，并与 她将在毕业论文期间指导本科生。Alyssa还将更多地关注 网络，特别是通过参加未来两年的多个科学会议。 最后，Alyssa将获得广泛的额外写作和口语经验，因为这些都是 她最需要改进的地方。因为她能把所有的时间都花在实验室工作上， 而不必教由于我的预算不足，我目前的MIRA的剩余一年 资金，Alyssa将更有可能在多个出版物和改进方面表现出色， 写作和口语，这将大大提高她作为一个独立的职业生涯取得成功的能力 调查员

Project Summary Alyssa will use molecular genetics and high resolution light microscopy to further advance her discovery that microtubules are an integral component of the C. elegans oocyte cortex, where they serve to stiffen the cortex and thereby limit the extent of membrane ingressions during oocyte meiotic cell division and polar body extrusion. Through additional training, Alyssa will acquire extensive new skills in molecular genetics (optogenetics, gene replacement and genome editing) and microscopy (near TIRF, Zeiss Airyscan, and Nikon Spinning Disk SoRa microscopy). She also will gain extensive experience in collaborative, interdisciplinary research with our Imaging Facility Dr. Adam Fries and senior research associates in my lab, and with the undergraduates that she will mentor during her thesis work. Alyssa also will focus more on networking, particularly by attending multiple science conferences over the next two years. Finally, Alyssa will gain extensive additional writing and speaking experience, as these are the areas in which she most needs improvement. By being able to devote all of her time to lab work, without having to teach due to my insufficient budget for the remaining year of my current MIRA funding, Alyssa will be far more likely to excel with multiple publications and improvements in writing and speaking that will greatly improve her ability to succeed in career as an independent investigator.