Advanced optics to unlock large field-of-view multiphoton imaging for neuroscience and beyond

项目总结 目前可用的光学元件限制了生物医学成像。特别是在神经生物学方面，标准显微镜 组件限制了神经元活动的视野和成像速度。通过先前对大脑的研究 倡议项目，我们已经开发了新的光学元件的方法，将视场扩大到5%到50% 比标准显微镜高出一倍，分辨率没有损失，同时保持了高成像速度。在这个项目中， 我们将开发三种新产品来进一步影响这一领域，使更好的成像系统，更高的质量 数据和新的实验。这三个产品是：(目标1)一种新的长工作距离空气目标，具有高 分辨率，(目标2)用于大视场成像的高性能扫描透镜，(目标3)角度倍增 用于大视场成像的模块，扫描速度不会降低。该公司将进行设计研究 和发展。该公司将与大学实验室合作，在 最先进的实验。该项目的结果将导致创新的新光学元件 将扩大神经生物学实验室成像系统的视野和吞吐量。

Project summary Currently available optical components limit biomedical imaging. In particular, in neurobiology, standard microscope components limit the field-of-view and speed of imaging of neuronal activity. Through prior work on BRAIN Initiative project, we have developed approaches for new optical components that expand the field-of-view 5 to 50 times over standard microscopes, with no loss in resolution, while retaining high imaging speed. In this project, we will develop three new products to further impact this field, enabling better imaging systems, higher quality data, and new experiments. The three products are: (Aim 1) a new long working distance air objective with high resolution, (Aim 2) a high performance scan lens for large field-of-view imaging, and (Aim 3) an angle-doubling module for large field-of-view imaging with no loss in scan speed. The company will perform the design studies and development. The company will partner with the university lab to validate and evaluate the products in state-of-the-art experiments. The results from this project will result in innovative new optical components that will expand the field-of-view and throughput of imaging systems in neurobiology labs.