Acquisition of Quant Studio 5 as replacement of aging thermocycler for diagnostic testing

项目摘要 威斯康星州兽医诊断实验室（WVDL）参与了样本 FDA Vet-LIRN分析，用于动物食品/药品紧急情况期间的监测 疫情爆发期间或大规模动物食品/药品紧急事件期间的应急能力。期间 在过去的几年里，我们已经实施了从狗饲料和粪便中培养沙门氏菌， 来自动物饲料的李斯特菌，培养物和来自狗粪便的直接空肠弯曲杆菌PCR。我们有 在E.大肠杆菌碳青霉烯类耐药基因PCR检测。此外，我们还有 我参与了Vet-LIRN或我们的人类健康合作伙伴的几项研究，包括 对感染SARS-CoV-2和C.从食用生食的犬中分离空肠 肉食。我们一直非常积极地支持SARS-CoV-2 PCR检测， 作为这些检测工作的一个组成部分，实时PCR检测来自阳性家庭的动物。 WVDL继续开发和验证新的PCR检测方法，以满足以下两种检测需求： 大型和小型动物。对于伴侣动物测试，我们与华盛顿大学密切合作， 庇护所医疗计划，以应对各地庇护所爆发的各种疾病， 国家本提案的具体要求是更换老化的ABI 7500 Fast 热循环仪与较新型号的Quant Studio 5，以帮助WVDL维持我们的能力， 分子检测和对动物食品/药品紧急情况或疾病爆发的关键反应。作为 作为Vet-LIRN实验室，我们随时准备应对这些紧急测试 需求

Project Abstract The Wisconsin Veterinary Diagnostic Laboratory (WVDL) has participated in sample analysis for the FDA Vet-LIRN, either for surveillance, during an animal food/drug emergency outbreak or during a large-scale animal food/drug emergency event for surge capacity. During the past years, we have implemented culture for Salmonella from dog feed and feces, culture of Listeria from animal feed, culture and direct Campylobacter jejuni PCR from dog feces. We had also investigated in E. coli carbapenem-resistant gene PCR assay. Additionally, we have participated in several investigations with Vet-LIRN or our human health partners including necropsy of a cat infected with SARS-CoV-2 and C. jejuni isolation from a dog consuming a raw meat diet. We have been very active in supporting SARS-CoV-2 PCR testing of exposed animals from positive households with real-time PCR as an integral part of these testing efforts. The WVDL continue to develop and validate new PCR assays to meet testing need for both large and small animals. For companion animals testing, we worked very closely with the UW shelter medicine program in responding to various disease outbreak in shelters across the country. The specific request for this proposal is to replace an aging ABI 7500 Fast thermocycler with a newer model of Quant Studio 5 to aid WVDL in maintaining our capacity in molecular testing and critical response to animal food/drug emergency or disease outbreak. As a Vet-LIRN laboratory, we are always stand ready to response to these emergency testing needs.