FDA Vet-LIRN Capacity-Building of Ohio ADDL to support bacterial identification of foodborne pathogens

这项来自俄亥俄州农业部（ODA）的建议是为了回应资金 美国食品药品监督管理局的机会公告（FOA）PAR-18-604 (FDA)兽医中心（CVM）兽医实验室调查和 反应网络（Vet-LIRN）。 ODA动物疾病诊断实验室（ADDL）的这一建议解决了 以下主要项目领域： 1.购买必要设备，以提高Vet-LIRN的诊断能力 食源性致病菌的个案调查。 a.我们正在申请资金以支持购买布鲁克MALDI Biotyper® Sirius RUO系统用于细菌鉴定。 2.针对 VPO。 a. MALDI-TOF技术允许高通量鉴定 以减少细菌分离的周转时间， 文化与隔离 此外，参与其他项目，如方法开发和/或 VPO确定的方法验证/改进和基质扩展工作 可列入能力建设基金。这项建议将保持能力 进行高通量样本的分子诊断测试 处理能力和数据分析， 减少由于设备故障或需要维修而导致的测试延迟。

This proposal from the Ohio Department of Agriculture (ODA) is in response to Funding Opportunity Announcement (FOA) PAR-18-604 from the Food and Drug Administration (FDA) Center for Veterinary Medicine (CVM) Veterinary Laboratory Investigation and Response Network (Vet-LIRN). This proposal from ODA’s Animal Disease Diagnostic Laboratory (ADDL) addresses the following key project areas: 1. Purchase of equipment needed to improve diagnostic capacity for Vet-LIRN case investigations of foodborne pathogens. a. We are requesting funds to support to the purchase of a Bruker MALDI Biotyper® Sirius RUO System for bacterial identification. 2. Conduct increased testing in response to a new need identified by the VPO. a. MALDI-TOF technology allows for high throughput identification of bacterial isolates to reduce turnaround time of bacterial culture and isolation. Additionally, participation in additional projects such as method development and/or method validation/improvement and matrix extension work as determined by the VPO may be included in the capacity-building funds. This proposal will maintain the capacity of our laboratory to conduct molecular diagnostic testing for high-throughput sample processing capability and data analysis by maintaining active service contracts to reduce any delays in testing due to equipment malfunctions or needed repairs.