Whole genome sequencing for outbreak investigations of foodborne pathogens and antimicrobial resistance studies for FDA Vet-LIRN

FDA CVM Vet-LIRN计划拨款提案 FOA#PAR-18-604 “VET-LIRN网络能力建设项目” 摘要 俄亥俄州农业部(ODA)的这项拨款提案是对 资助机会公告(FOA)#PAR-18-604，来自食品和药物管理局 美国食品和药物管理局兽医中心(CVM)研究室 兽医实验室调查和反应网络(VET-LIRN)。该项目是 题为《全基因组测序用于食源性致病菌暴发调查》 FDA Vet-LIRN的抗菌素耐药性研究“。 来自oda的动物疾病诊断实验室(ADTL)的这项建议解决了 以下是主要项目领域： 1.提高实验室开展病原体药敏试验的能力 通过为更新或购买更多设备提供资金。 2.采购AMR测试工作或方法改进所需的用品。 3.购买处理Vet-LIRN食源性样本的液体处理系统 病原体病例调查。 4.根据VPO确定的新需求进行更多的测试。 5.参与多个实验室的盲测，以验证新开发的Vet-LIRN 网络方法。 ADSL还准备应对新兴监管科学的新发展 以及潜在研究的紧迫或新领域。几个附加文件 包括内部能力测试(PT)结果(研究计划附录)，以及 AAVLD认证证书(研究计划附录)随附 这个

FDA CVM Vet-LIRN PROGRAM GRANT PROPOSAL FOR FOA # PAR-18-604 “Vet-LIRN Network Capacity-Building Projects” ABSTRACT This grant proposal from the Ohio Department of Agriculture (ODA) is in response to Funding Opportunity Announcement (FOA) # PAR-18-604 from the Food and Drug Administration’s (FDA) Center for Veterinary Medicine (CVM) Office of Research Veterinary Laboratory Investigation and Response Network (Vet-LIRN). The project is titled “Whole genome sequencing for outbreak investigations of foodborne pathogens and antimicrobial resistance studies for FDA Vet-LIRN”. This proposal from ODA’s Animal Disease Diagnostic Laboratory (ADDL) addresses the following key project areas: 1. Increasing the capacity of laboratories conducting pathogen susceptibility testing by funding the updating or purchasing of additional equipment. 2. Procurement of supplies needed for AMR testing efforts or method improvement. 3. Purchase of liquid handling system for handling of samples for Vet-LIRN foodborne pathogen case investigations. 4. Conduct increased testing in response to a new need identified by the VPO. 5. Participation in multi-laboratory blinded tests to validate newly developed Vet-LIRN Network methods. The ADDL is also poised to address new developments in emerging regulatory sciences as well as urgent or novel areas for potential research. Several additional documents including the internal Proficiency Testing (PT) results (appendix of Research Plan), and certificate of AAVLD accreditation (appendix of Research Plan) are provided along with the