Whole genome sequencing for outbreak investigations of foodborne pathogens and antimicrobial resistance studies for FDA Vet-LIRN

FDA CVM Vet-LIRN动物授权提案 FOA # PAR-18-604 “Vet-LIRN网络能力建设项目” 摘要 这项来自俄亥俄州农业部（ODA）的拨款提案是为了回应 Food and Drug的资助机会公告（FOA）# PAR-18-604 美国食品药品监督管理局（FDA）兽药中心（CVM）研究办公室 兽医实验室调查和反应网络。该项目 标题为“全基因组测序用于食源性病原体爆发调查， FDA Vet-LIRN的抗生素耐药性研究”。 ODA动物疾病诊断实验室（ADDL）的这一建议解决了 以下主要项目领域： 1.提高实验室进行病原体敏感性测试的能力 通过资助更新或购买额外设备。 2.采购AMR检测工作或方法改进所需的供应品。 3.购买用于处理Vet-LIRN食源性样本的液体处理系统 病原体病例调查。 4.根据VPO确定的新需求进行更多测试。 5.参与多实验室盲法测试，以验证新开发的Vet-LIRN 网络方法。 ADDL还准备应对新兴监管科学的新发展 以及潜在研究的紧迫或新颖领域。若干补充文件 包括内部能力验证（PT）结果（研究计划附录），以及 AAVLD认证证书（研究计划附录）随附沿着 的

FDA CVM Vet-LIRN PROGRAM GRANT PROPOSAL FOR FOA # PAR-18-604 “Vet-LIRN Network Capacity-Building Projects” ABSTRACT This grant proposal from the Ohio Department of Agriculture (ODA) is in response to Funding Opportunity Announcement (FOA) # PAR-18-604 from the Food and Drug Administration’s (FDA) Center for Veterinary Medicine (CVM) Office of Research Veterinary Laboratory Investigation and Response Network (Vet-LIRN). The project is titled “Whole genome sequencing for outbreak investigations of foodborne pathogens and antimicrobial resistance studies for FDA Vet-LIRN”. This proposal from ODA’s Animal Disease Diagnostic Laboratory (ADDL) addresses the following key project areas: 1. Increasing the capacity of laboratories conducting pathogen susceptibility testing by funding the updating or purchasing of additional equipment. 2. Procurement of supplies needed for AMR testing efforts or method improvement. 3. Purchase of liquid handling system for handling of samples for Vet-LIRN foodborne pathogen case investigations. 4. Conduct increased testing in response to a new need identified by the VPO. 5. Participation in multi-laboratory blinded tests to validate newly developed Vet-LIRN Network methods. The ADDL is also poised to address new developments in emerging regulatory sciences as well as urgent or novel areas for potential research. Several additional documents including the internal Proficiency Testing (PT) results (appendix of Research Plan), and certificate of AAVLD accreditation (appendix of Research Plan) are provided along with the