Monitoring of antimicrobial resistance in bacteria of veterinary importance

项目摘要/摘要 康奈尔大学纽约兽医诊断中心/康奈尔动物健康诊断中心 是一家提供全方位服务的多学科兽医诊断实验室，也是东北地区唯一的此类实验室。 美国。2022年，AHDC收到了超过28万份客户申请。细菌科有 MALDI-TOF分析仪，Sensititer系统，veraTREK®血培养仪器，BSL3可升级 工作空间和16S DNA测序能力，使其成为提供全方位服务的细菌学实验室。另外， AHDC有能力进行全基因组测序。美国食品和药物管理局细菌学实验室 2022年对临床样本、组织、血清、细菌分离、环境、 食品和饲料样本，包括5,000多个抗菌敏感板。阿赫德中心已经与 FDA并多次在宠物食品和动物饲料污染调查中发挥积极作用 在过去的二十年里。我们希望继续参与FDA的Vet-LIRN计划和其他 网络实验室可： 1.继续参与VPO指定样本分析和监督活动，以促进 动物健康和福利，并增加兽医-LIRN网络的紧急援助能力 以及大规模的疫情检测。 2.通过制定和使用标准化方法，提供支持监管行动的分析数据； 设备、平台和报告方法。继续参加由 VPO，应VPO的要求继续调查消费者报告的案件，并继续 改进和实施VPO指定的标准化质量管理体系。 3.继续参与小规模抗生素药敏试验研究，以应对新出现的问题 抗菌素耐药性问题，作为FDA Vet-LIRN AMR监测的来源实验室 收集和提交细菌分离物和相关的抗菌药敏感性数据的项目。

Project Summary/Abstract The NYS Veterinary Diagnostic Center/Cornell Animal Health Diagnostic Center at Cornell University (AHDC) is a full-service multidisciplinary veterinary diagnostic laboratory and the only of its kind in the northeaster United States. The AHDC received over 280,000 client accessions in 2022. The Bacteriology section has MALDI-TOF analyzers, Sensititer systems, veraTREK® blood culture instruments, a BSL3 upgradable workspace, and 16S DNA sequencing capabilities, making it a full-service bacteriology laboratory. Additionally, the AHDC has the capacity to perform whole genome sequencing. The Bacteriology laboratory of the AHDC performed over 100,000 tests in 2022 on clinical samples, tissues, serum, bacterial isolates, environmental, food, and feed samples, including over 5,000 antimicrobial sensitivity panels. The AHDC has partnered with the FDA and played an active role in pet food and animal feed contamination investigations many times over the past two decades. We hope to continue our participation with the FDA Vet-LIRN program and other network laboratories to: 1. Continue to participate in VPO designated sample analyses and surveillance activities to promote animal health and welfare and add to the Vet-LIRN Network’s surge capacity to assist in emergency and large-scale outbreak testing. 2. Provide analytical data to support regulatory actions by developing and using standardized methods, equipment platforms, and reporting methods. Continuing to participate in proficiency testing provided by the VPO, continue investigating consumer reported cases as requested by the VPO, and continuing to improve and implement standardized quality management systems as designated by the VPO. 3. Continue to participate in small-scale antibiotic susceptibility testing studies to address emerging antimicrobial resistance issues, acting as a source laboratory for the FDA Vet-LIRN AMR monitoring project to collect and submit bacterial isolates and associated antimicrobial susceptibility data.