Prefrontal cortical circuit reorganization during immediate memory consolidation

项目总结 学习和记忆是基本的认知功能，使我们能够从周围的环境中学习 我们。记忆是储存和回忆日常生活中必不可少的信息的能力。一项不可或缺的财产 记忆是它的稳定存储，允许在更长的时间段内回忆起记忆。然而， 记忆如此长时间稳定存储的生理机制仍有待研究 已澄清。记忆的形成包括两个阶段，编码和巩固。编码后， 巩固过程将这些神经元的痕迹固化成持久的记忆。整合是一种 循序渐进的过程，从立即巩固开始，随着时间的推移，变成长期巩固。 海马体(HPC)是大脑中记忆形成和即刻巩固的基础区域。AS 整合继续，HPC将记忆卸载到大脑皮层，最终变得独立于 高性能计算机。内存的HPC整合涉及称为尖峰波的高度同步的网络事件 波纹(SWR)。在SWR期间，表示特定体验的内存跟踪将在 压缩方式，这被认为是通过突触巩固这些神经元表征 可塑性。然而，尚不清楚在短波反射过程中，来自HPC的信息是如何传递到大脑皮层的 立即整合。与记忆有关的一个皮质区域是前额叶皮质(PFC)。这两个 HPC和PFC参与了空间记忆，使得研究HPC迁移成为一种理想的行为 在立即合并期间向PFC提供信息。SWR和局部大脑皮层各自的贡献 即刻记忆巩固的电路动力学仍不清楚。此外，电路和蜂窝 对即时合并过程中的变化还没有完全了解。这项提案针对的正是这些问题 通过1)直接测试SWR是否向PFC传输即时整合所需的信息；2) 识别即时固结基础上的SWRS期间的PFC神经元组装修改；以及3) 阐明调节这些神经元组装变化的局部PFC电路机制 立即整合。这项提案的目的将直接测试HPC和 在SWR期间发生的PFC对于即时内存整合以及 PFC在记忆整合过程中进化。这项工作与NIMH的使命一致，即了解 通过研究脑区之间的相互作用在系统水平上研究学习和记忆的机制 这可能是这些功能的基础。

PROJECT SUMMARY Learning and memory are fundamental cognitive functions that allow us to learn from the environment around us. Memory is the ability to store and recall information essential to everyday life. An indispensable property of memory is its stable storage, allowing for the recall of memories over extended periods of time. Yet the physiological mechanisms underlying the stable storage of memories for such a long-lasting time remain to be elucidated. Memory formation involves two stages, encoding, and consolidation. After encoding, the consolidation process solidifies these neuronal traces into long-lasting memories. Consolidation is a progressive process, starting with immediate consolidation that, over time, becomes long-term consolidation. The hippocampus (HPC) is a brain region fundamental to memory formation and immediate consolidation. As consolidation continues, the HPC offloads memories to the cortex, eventually becoming independent of the HPC. The HPC consolidation of memories involves a highly synchronous network event called a sharp-wave ripple (SWR). During SWRs, memory traces that represent a particular experience are replayed in a compressed manner, which is thought to consolidate these neuronal representations through synaptic plasticity. Yet, it is unknown how information from the HPC is transferred to the cortex during SWRs during immediate consolidation. A cortical region also involved in memory is the prefrontal cortex (PFC). Both the HPC and PFC are involved in spatial memory, making it an ideal behavior to investigate the transfer of HPC information to the PFC during immediate consolidation. The respective contribution of SWRs and local cortical circuit dynamics for immediate memory consolidation remains unknown. Furthermore, the circuit and cellular changes during immediate consolidation are not fully understood. This proposal targets these exact questions by 1) directly testing whether SWRs transfer information to the PFC necessary for immediate consolidation; 2) identifying the PFC neuronal assembly modifications during SWRs underlying immediate consolidation; and 3) elucidating the local PFC circuitry mechanisms that regulate these neuronal assembly changes during immediate consolidation. The aims of this proposal will directly test whether communication between HPC and PFC that occurs during SWRs is crucial for immediate memory consolidation and how local processes in the PFC evolve during memory consolidation. This work aligns with NIMH’s mission to understand the mechanisms of learning and memory at a system level by investigating the interactions between brain regions that might underlie these functions.