Cardiorenal and Metabolic Diseases Research Center

项目总结/摘要 心血管（CV）、肾脏和代谢疾病在美国非常普遍，尤其是在密西西比。我们 在密西西比大学建立了肾脏和代谢疾病研究中心 医学中心汇集了基础，临床和人口科学家的多学科小组， CV、肾脏和代谢疾病。了解这些慢性疾病之间的复杂关系 需要基础、临床和人口科学家的共同努力， 综合研究方法和促进发现的最先进技术。分子 CV、肾脏和代谢疾病相互依赖的基础已经经典地通过基因组学进行了研究， 转录组学和蛋白质组学平台，用于评估DNA序列以及RNA和蛋白质水平 表情虽然从这些研究中产生的数据是翔实的，但复杂生物系统的调节 还需要了解蛋白质的活性。蛋白激酶是控制蛋白活性网络的核心 并连接几个调控元件，包括转录因子、伴侣蛋白和结构蛋白。的 激酶组是指完整蛋白激酶组的广泛活性。最近有几种方法 已开发用于根据蛋白激酶的巨大活性来研究激酶组。这些新技术之一 是PamChip，其使用数百个~13个残基长的报告肽，这些报告肽被磷酸化， 来源于细胞培养物、组织或临床活检的样品。磷酸化的程度是在 使用荧光抗体进行实时检测。该技术包含在Pamgene的PAMStation 12中。 此设备补充申请是为CMDRC购买PAMStation 12的资金。收购 这种最先进的系统将允许母体COBRE的研究人员确定激酶组的作用 在CV、肾脏和代谢疾病领域。这种新设备还将提高 实验方法，并加强未来的NIH赠款从CMDRC研究人员以及其他 UMMC的研究人员，包括由其他NIGMS资助的IDEA中心支持的研究人员。CMDRC一直在 是密西西比心肾和代谢疾病研究显著扩展的主要驱动力。这个大 设备赠款将扩大和确保我们努力发展一个高生产力的CMDRC的可持续性 致力于通过研究、发现和创新来改善生活。

PROJECT SUMMARY/ABSTRACT Cardiovascular (CV), renal and metabolic diseases are highly prevalent in the U.S., especially in Mississippi. We established the Cardiorenal and Metabolic Diseases Research Center (CMDRC) at the University of Mississippi Medical Center to bring together a multidisciplinary group of basic, clinical and population scientists working on CV, renal, and metabolic diseases. Understanding the complex relationship between these chronic diseases requires the combined efforts of basic, clinical and population scientists using innovative multidisciplinary integrative approaches to research and state-of-the-art technologies that facilitate discovery. The molecular bases for interdependence of CV, renal, and metabolic diseases have classically been studied via genomics, transcriptomics, and proteomics platforms that assess DNA sequences and levels of RNA and protein expression. While data generated from these studies are informative, regulation of complex biological systems also requires understanding protein activity. Protein kinases are central to governing protein activity networks and link several regulatory elements, including transcription factors, chaperones, and structural proteins. The kinome refers to the broad-based activity of the complete set of protein kinases. Several methods have recently been developed to study the kinome in light of the vast activity of protein kinases. One of these new technologies is the PamChip which uses hundreds of ~13 residue-long reporter peptides which are phosphorylated with samples derived from cell cultures, tissues, or clinical biopsies. The degree of phosphorylation is measured in real-time using fluorescent antibodies. This technology is encompassed in the PAMStation 12 from Pamgene. This equipment supplement application is for funds to purchase a PAMStation 12 for the CMDRC. The acquisition of this state-of-the-art system will allow investigators of the parent COBRE to determine the role of the kinome in the areas of CV, renal, and metabolic diseases. This new equipment will also increase the sophistication of experimental approaches and strengthen future NIH grants from CMDRC investigators as well as other investigators at UMMC, including those supported by other NIGMS funded IDeA centers. The CMDRC has been a major driver for marked expansion of cardiorenal and metabolic diseases research in Mississippi. This large equipment grant will amplify and ensure sustainability of our efforts to develop a highly productive CMDRC dedicated to improving lives through research, discovery, and innovation.