The Human Lung BioMolecular Multi-Scale Atlas Program (HuBMAP-Lung)

项目摘要/摘要 总体研究计划 人类肺生物分子多尺度图谱计划(HuBMAP-ROUNG) 人类生物分子图谱计划(HuBMAP)正在进入生产阶段，重点是 循序渐进、高灵敏度和特异性的分析，优先进行空间生物分子分析。我们的组织图谱 中心(TMC)计划将重点放在一个器官系统上，由两个肺和相关的呼吸道组成 (气管到肺泡)，为简单起见，我们称之为“肺”。我们的TMC将生成、标准化和验证 来自高内容、高通量成像和组学技术的大量数据，以产生系统的人类 高分辨率的肺组织图。我们将从长远来看地图集，它是2D和3D地图的集合 包含图像、表格数据、有关不同分辨率的多个位置的事实以及名为 以类似于纬度和经度的位置网格的坐标为关键点的对象，以地图形式呈现 人类肺部的所有显著特征。为了完成这项任务，我们中心汇集了 来自五个机构的调查人员，罗切斯特大学(URMC)，加州大学圣地亚哥分校 (UCSD)、太平洋西北国家实验室(PNNL)、华盛顿大学(UW)和北方大学 卡罗莱纳在教堂山(北卡罗来纳大学)，每个人都有互补的以肺为重点的学术兴趣、知识和 财团网络联系，并具有国际公认的肺部和最先进的专业知识 生物分子技术。我们在第一阶段向HuBMAP数据门户提供的肺部数据侧重于 单核RNA测序，染色质可用性和开始空间转录。除了……之外 在这些数据类型中进一步增加人类多样性的表现，我们的TMC的下一阶段将成为主要的 努力确定不仅由基因表达而且由基因表达定义的细胞和基质的空间组织 蛋白质、蛋白质修饰、脂类和精选代谢物对特定的细胞功能至关重要 实用的利基市场。鉴于财团内外的全球调查工作的价值得到认可， TMC总体组件的具体目标集中在 TMC，在所有HuBMAP组件之间，并在国家和国际研究人员和 财团。

Project Summary/Abstract Overall Research Plan The Human Lung Biomolecular Multi-Scale Atlas Program (HuBMAP-Lung) The Human Biomolecular Atlas Program (HuBMAP) is entering the production phase, with an emphasis on progressive, high sensitivity and specificity assays, prioritizing spatial biomolecular analysis. Our Tissue Mapping Center (TMC) proposal will focus on one organ system, comprised of two lungs and related respiratory tract (trachea to alveoli) that for simplicity we refer to as the “Lung”. Our TMC will generate, standardize, and validate extensive data from high content, high-throughput imaging and `omics technology to produce systematic human lung tissue maps at high resolution. We will take a long view of an Atlas, as a collection of 2D and 3D maps containing images, tabular data, facts about multiple locations at varying resolution, and indexes of named objects keyed to coordinates of a locational grid analogous to latitude and longitude, to cartographically present the whole range of salient features of the human Lung. To accomplish this task, our center brings together Investigators from five institutions, the University of Rochester (URMC), University of California at San Diego (UCSD), Pacific Northwest National Laboratory (PNNL), University of Washington (UW), and University of North Carolina at Chapel Hill (UNC), each with complementary lung-focused academic interests, knowledge, and inter- consortia network connections and with internationally recognized expertise in lung and state-of-the-art biomolecular technologies. Our Lung data provided to the HuBMAP Data Portal in Phase I has been focused to single nucleus RNA-sequencing, chromatin availability and beginning spatial transcriptomics. In addition to further increasing representation of human diversity in these data types, the next phase of our TMC turns primary effort toward determining spatial organization of cells and matrices defined by not only gene expression but also proteins, protein modifications, lipids and select metabolites critical to specific cell function within anatomical and functional niches. With the recognized value of global investigative efforts within and outside the Consortium, the Specific Aims of the TMC Overall Component concentrate on Communication and Collaboration within the TMC, between all HuBMAP components and synergistically among national and international researchers and Consortia.