IMPROVING THE USE OF ENZYMES FOR SYNTHESIS

描述：这个项目的目的是探索新的合成 有机溶剂中的酶催化提供的机会， 特别是对于不对称(和其他选择性的)的发展 转化及其在生物医学生产中的应用 相关化合物。成功使用酶的一个缺点是 要有效地达到上述目的，很难预测 在给定的有机溶剂中的给定反应的结果。要做到这一点 目的有机溶剂对酶的三级结构的影响 首先被确定。这将通过“映射”酶的 活性中心周围的三级结构，在不同的有机溶剂中。 第二，对酶对映体选择性的溶剂依赖性的研究 扩展到固定化、交联和聚合物包埋的酶 准备工作。最终目标是找到一种系统，在其中 酶的对映体选择性可以在任何有机化合物中合理地预测 任何给定底物的溶剂。有机溶剂中的酶催化将 成为合成手性生物活性的有力工具 化合物。

DESCRIPTION: The aim of this project is to explore novel synthetic opportunities afforded by enzymatic catalysis in organic solvents, particularly for the development of asymmetric (and otherwise selective) transformations, and for their utilization in the production of biomedically relevant compounds. One of the draw-backs of using enzymes successfully and efficiently for the purpose mentioned above is the difficulty to predict the outcome of a given reaction, in a given organic solvent. To achieve this aim the effect of organic solvents on the enzyme's tertiary structure will be first ascertained. This will be accomplished by "mapping" the enzyme's tertiary structure around the active site, in different organic solvents. Second, the study of solvent dependence of enzyme enantioselectivity will be extended to immobilized, cross-linked, and polymer-encapsulated enzyme preparations. The ultimate goal is to find a system in which the enantioselectivity of an enzyme can be rationally predicted in any organic solvent with any given substrate. Enzyme catalysis in organic solvents will became a powerful tool for the syntheses of chiral biologically active compounds.