CHEMICAL SYNTHESIS OF RNA VIA NOVEL STRATEGY

对RNA结构成分、核酶和 其他RNA功能产生了对可靠方法的需求， 合成小RNA寡核苷酸（少于50个核苷酸）。 尽管RNA合成可以在某些应用中实现， 由知识渊博的专家通过生物化学方法，例如， 转录，或化学，例如5 '-二甲氧基三苯甲基-2'-甲硅烷基 在化学上，我们确实需要改进RNA的合成 方法. RNA的合成将更加强大， 生物学家很容易接触到，他们在推动 健康相关研究的进展。 合成的最终加工 RNA必须在温和的无菌条件下进行， 装卸. 我们的最终加工是在温和的水溶液中完成的。 条件下，pH 3，55埃C，10分钟。 的目的 这项研究有两个方面：（1）确定可以进行这种化学反应 商业上可获得的，即研究和开发新的 保护基团，使它们能够大规模地 经济的合成，（2）建立这一普遍效用 化学合成各种RNA寡核苷酸， 由多位合作者进行评估。 实现这些目标 将满足研究界的市场需求， 获取RNA寡核苷酸。

The growing interest in RNA structural components, ribozymes and other RNA functions has generated a need for reliable methods of synthesizing small RNA oligonucleotides (less than 50 nucleotides). Although RNA synthesis can be accomplished for some applications by knowledgeable specialists via either biochemical methods, e.g. transcription, or chemically, e.g. 5'-dimethoxytrityl-2'-silyl chemistry, there is a definite need for improved RNA synthesis methods. RNA synthesis would be considerably more powerful if readily accessible to biologists, who play central roles in driving advances in health-related research. Final processing of synthesized RNA must be done under mild, sterile conditions with minimal handling. Our final processing is accomplished under mild aqueous conditions, pH 3, 55 angstroms C for 10 minutes. The purpose of this study is twofold: (1) establish that this chemistry can be made commercially available, i.e. research and develop the novel protecting group so that they are amenable to large scale economical synthesis, (2) establish the general utility of this chemistry by synthesizing a variety of RNA oligonucleotides to be evaluated by several collaborators. Accomplishment of these goals would sastify the market need in the research community for ready access to RNA oligonucleotides.