BIOELASTIC MATERIAL TO PREVENT POSTLAMINECTOMY ADHESION

这项第一阶段的SBIR建议为生物弹性材料开发 预防椎板切除术后粘连是对 NICHD， 国家康复研究中心(NCMRR)征集 (SBIR) 96-2)用于“将增强功能的材料的开发” 和 身体残障人士的生活质素。“ 物理 腰椎间盘突出症致残 构成了 第三种最常见的工人伤残情况 在 美国将改善腰椎板切除术的疗效 会是 为了提高生活质量，恢复许多人的功能 数以百万计的残疾美国人。 特殊的生物弹性材料--弹性蛋白质基聚合物 由重复的多肽序列组成，用于展示 非同寻常的生物相容性；它们不会形成纤维性胶囊 植入时形成；它们不支持细胞附着，也不支持 这个 对血液蛋白质的吸附，可以预期它们会 慢慢地 降解产物为天然氨基酸。 预防粘连的方法探讨 椎间后 椎间盘摘除涉及：以两种弹性蛋白为主的发展 聚合物，它们的高分子量微生物生产和 高 在去除内毒素的情况下，样品制备 的 弹性片材和粘弹性凝胶在兔的应用 AS 腰椎区腰椎间盘摘除动物模型的建立 这个 覆盖中线硬膜囊的床单和保护 退出 神经根瘢痕形成及硬膜外瘢痕形成的评价 和 炎症和疼痛标记物的存在，如白细胞介素1 和 P物质在组织学上的抗体探针研究 横断面。

This Phase I SBIR proposal to develop bioelastic materials for the prevention of post-laminectomy adhesions is in response to an NICHD, National Center for Rehabilitation Research (NCMRR) solicitation (SBIR 96-2) for "the development of materials that will enhance function and quality of life of individuals with physical disabilities." The physical disability resulting from intervertebral disc herniation constitutes the third most common condition for which worker disability is granted in the U.S. To improve the outcome of intervertebral disc laminectomy would be to improve the quality of life and rehabilitate function for many millions of disabled Americans. The particular bioelastic materials, elastic protein-based polymers comprised of repeating peptide sequences, to be used to exhibit extraordinary biocompatibility; they do not elicit fibrous capsule formation when implanted; they do not support cell attachment nor the adsorption of blood proteins that do, and they can be expected to slowly degrade with breakdown products being natural amino acids. The approach to the prevention of adhesions due to post-intervertebral discectomy involve: the development of two elastic protein-based polymers, their microbial production at high molecular weights and high yields with removal of endotoxins, the sample preparation in terms of elastic sheets and viscoelastic gels, the utilization of the rabbit as the animal model for discectomy at the lumbar spine region using the sheets to cover the midline dural sac and the gel to protect the exiting nerve roots from scarring, and the evaluation of peridural scarring and the presence of inflammatory and pain markers such as interleukin-1 and substance P by means of antibody probes on the histological sections.