PEPTIDES BASED ON ACTIVE SITES OF ACTIN BINDING PROTEINS

描述：建议继续并扩大合作 拉脱维亚一个杰出的肽和有机合成实验室 和波士顿一个杰出的细胞骨架实验室潜在肌动蛋白 或多磷酸肌醇脂质（PPI）结合肽将被合成 基于通过遗传学或基因工程鉴定的各种蛋白质的序列， 生化数据。一些肽的结构将通过以下方式计算： 里加的分子模型及其对脂质的生化影响， 肌动蛋白结合蛋白和完整的细胞将在波士顿进行研究。 这些研究将扩大到包括荧光肽， 保留未标记类似物的PPI结合特性。其中一 肽显示出穿过质膜的不寻常的特性 活细胞。该肽具有潜在的研究应用价值 用于识别或破坏PPI体内或临床定位的工具， 一种药物载体进入特定的细胞类型。这些 可能性将使用在里加合成的衍生物进行测试， 在波士顿展示了通过组装在非常低的浓度下形成凝胶 变成棒状胶束。这些肽凝胶是理想的材料， 通过流变学方法进行研究，并具有在药物中应用的潜力 递送或抗原呈递。

DESCRIPTION: What is proposed is to continue and expand a collaboration between an outstanding peptide and organic synthesis laboratory in Latvia and an outstanding cytoskeleton laboratory in Boston. Potential actin or polyphosphoinositide lipid (PPI)-binding peptides will be synthesized based on the sequences of various proteins identified by genetic or biochemical data. The structures of some peptides will be calculated by molecular modeling in Riga and their biochemical effects on lipids, actin binding proteins, and intact cells will be studied in Boston. These studies will be expanded to include fluorescent peptides shown to retain the PPI-binding properties of unlabeled analogs. One of these peptides exhibits the unusual property of crossing the plasma membrane of living cells. This peptide has potential applications as a research tool to identify or disrupt PPI localization in vivo or clinically as a carrier of pharmaceutical agents into specific cell types. These possibilities will be tested using derivatives synthesized in Riga and shown in Boston to form gels at very low concentration by assembling into rodlike micelles. These peptide gels are ideal materials to be studied by rheologic methods and have potential for application in drug delivery or antigen presentation.