ANALYSIS OF REGULATION OF MUCIN GENE EXPRESSION

（根据研究者的具体目标改编） 改进的应用是：1）从大鼠中分离全长粘蛋白cDNA， 人气道库;从这些确定的一级结构 粘蛋白核心蛋白。 2)利用大鼠粘蛋白cDNA，分离 来自大鼠基因组文库同源基因，并鉴定其上游（或 其他）调控元件的转染分析 变种人 3)确定这些调节元件结合的程度 使用迁移率改变和DNA酶I保护测定的核蛋白， 4)建立大鼠呼吸道粘蛋白过度分泌模型， 粘蛋白生物合成的转录控制是否被上调， 大鼠粘蛋白cDNA在北方杂交和核运行分析。

(Adapted from the investigator's specific aims) The specific aims of this revised application are: 1) Isolate full length mucin cDNAs from rat and human airway libraries; from these determine the primary structure of mucin core protein in both species. 2) Using the rat mucin cDNA, isolate the cognate gene from a rat genomic library and identify upstream (or other) regulatory elements using transfection analysis of deletion mutants. 3) Determine the extent to which these regulatory elements bind nuclear proteins using mobility shift and dNase I protection assays and 4) Establish a rat model of respiratory mucin overproduction and determine whether transcriptional control of mucin biosynthesis is upregulated using a rat mucin cDNA in northern hybridization and nuclear run-on assays.