MODULATION OF A NEURONAL OSCILLATOR

这项提议的广泛的、长期的目标是了解神经元 动物处理感官信息的机制， 产生适应性行为。 特别令人感兴趣的是动物是如何产生 使用有限的运动回路的不同行为。 拟定研究 旨在阐明各种神经机制的基础上， 电行为所执行的电鱼，Hypopomus，在 求爱和攻击 最近发现， 本发明的电鱼的制备方法， 通过将L-谷氨酸盐微离子电渗到脑内的一个小区域中， 间脑，前起搏器核。 前两个具体目标是 了解不同的行为是如何在这个小小的大脑中组织起来的 结构 生理记录和解剖标记将 在体内制备中进行。 前起搏器核投射到 最后的运动核，起搏核，并调节其规则 振荡以产生电行为。 以下两个具体 目的是了解起搏核如何产生复杂的运动， 产出 尽管它的结构非常简单， 在组成神经元之间的耦合中，起搏核能够 在来自调制器的调制输入下生成各种输出， 起搏前核 体内制剂也将用于 诱导行为时起搏神经元的细胞内渗透 通过刺激前起搏器核 这些实验的结果将有助于理解 中枢神经系统如何调节不同类型的行为， 单神经元水平，以及如何调节看似刚性的网络， 产生不同的脊椎动物行为模式。

The broad, long-term objective of this proposal is to understand neuronal mechanisms by which animals process sensory information and accordingly generate adaptive behavior. Of particular interest is how animals generate diverse behaviors using limited sets of motor circuits. The proposed study is designed to elucidate the neuronal mechanisms underlying a variety of electrical behaviors performed by an electric fish, Hypopomus, during courtship and aggression. It was recently discovered that a variety of behaviors can be induced in a curarized preparation of this electric fish by microiontophoresis of L-glutamate into a small region in the diencephalon, the prepacemaker nucleus. The first two specific aims are to understand how different behaviors are organized in this small brain structure. Physiological recordings and anatomical labeling will be performed in in vivo preparations. The prepacemaker nucleus projects to the final motor nucleus, the pacemaker nucleus, and modulates its regular oscillation to generate electrical behaviors. The following two specific aims are to understand how the pacemaker nucleus generates complex motor outputs. Despite its very simple organization and the rigid electrical coupling between constituent neurons, the pacemaker nucleus is capable of generating a variety of outputs under the modulatory inputs from the prepacemaker nucleus. In vivo preparations will also be used for intracellular penetration of pacemaker neurons while behaviors are induced by stimulation of the prepacemaker nucleus. The results of these experiments will contribute to the understanding of how the central nervous system regulates different types of behavior at the single-neuron level and how seemingly rigid networks can be modulated to generate different patterns of vertebrate behavior.