PARASAGITTAL ORGANIZATION OF CEREBELLAR CORTEX

小脑皮质的矢状面旁组织 使用多种方法进行了演示，包括形态学、 生物化学和生理学方法。这些研究，在众多的 物种，说明了这个组织的明显共性 跨物种的图式，并暗示其在 小脑在运动控制、协调和执行中的作用 行为。在同一只动物身上分析这些模式是至关重要的。 为了确定是否存在潜在的么正 小脑的组织模式。最近的研究表明 攀爬纤维传入系统起源于 下层橄榄色复合体，与分布有共同边界 浦肯野细胞特异性抗原Zebrin 1的进一步研究 小鼠表现出与斑马素相同的矢状面旁间隔 1抗原和水解酶5‘-核苷酸酶。这些研究 建议对这三种不同的功能进行通用划分 小脑异质性。在本应用程序中，我们建议在 这些发现并继续我们对矢状面旁的分析 通过检查其他专业的组织来进行划分 传入系统、苔藓纤维和小脑皮质传出。 为此，将在啮齿动物身上进行以下研究。 (1)分析苔藓纤维传入的拓扑关系 到小脑皮层。这种来自脊髓的投射， 外侧网状核、脑桥基底核、前庭核、 将分析外楔状核和三叉神经核团 利用双重顺行标记范例。(2)比较 这些苔藓纤维投射到 斑马蛋白1抗原的分布。这将通过以下方式实现 顺行示踪剂和免疫组织学联合分析 同样的动物。(3)分析浦肯野细胞的传出投射。 通过逆行示踪分析确定的小脑皮质，以及 将这与Zebrin 1抗原的分布进行比较。这些 研究将提供基本数据，这些数据将显著增加 我们对小脑异质性的理解，从而有助于测试 关于这些异质性的共同蓝图的假设。这个 最终目标是更全面地理解 小脑及其周围组织的结构和功能组织 对正常运动行为和异常运动行为的影响 由小脑疾病引起。

The parasagittal organization of the cerebellar cortex has been demonstrated using numerous methodologies, including morphological, biochemical and physiological approaches. These studies, in numerous species, illustrate the apparent commonality of this organizational schema across species and allude to its importance in the function of the cerebellum in the control, coordination and execution of motor behavior. It is essential to analyze these patterns in the same animal in order to determine whether there is an underlying unitary organizational schema to the cerebellum. Recent studies demonstrate that the climbing fiber afferent system, which originates from the inferior olivary complex, share common boundaries with the distribution of the Purkinje cell specific antigen Zebrin 1. Additional studies in the mouse demonstrate identical parasagittal compartments for the Zebrin 1 antigen and the hydrolytic enzyme 5'-nucleotidase. These studies suggest a common compartmentation for these three different features of cerebellar heterogeneity. In this application, we propose to build on these findings and continue our analysis of parasagittal compartmentation by examining the organization of the other major afferent system, mossy fibers, and the cerebellar cortical efferents. Toward this end the following studies will be conducted in the rodent. (1) Analyze the topographic relationship between mossy fiber afferents to the cerebellar cortex. Such projections from the spinal cord, lateral reticular nucleus, basilar pontine nucleus, vestibular nuclei, external cuneate nucleus and trigeminal nuclei will be analyzed utilizing a double anterograde labeling paradigm. (2) Compare the topographic organization of these mossy fiber projections to the distribution of the Zebrin 1 antigen. This will be accomplished by combined anterograde tracer and immunohistological analysis within the same animal. (3) Analyze the efferent projection of Purkinje cells in the cerebellar cortex, as determined by retrograde tracer analysis, and compare this with the distribution of the Zebrin 1 antigen. These studies will provide essential data which will significantly increase our understanding of cerebellar heterogeneity and thereby help test the hypothesis of a common blueprint for these heterogeneities. The ultimate goal being to develop a more complete understanding of the structural and functional organization of the cerebellum and its influence on normal motor behavior, and abnormal motor behaviors which result from cerebellar disease.