PARASAGITTAL ORGANIZATION OF THE CEREBELLAR CORTEX

小脑皮质的一些传入和传出投射 被显示为一系列的副矢状面定向区域。这个 “小脑模块”或“微区”的概念被提出为 小脑的基本功能单位，它调节一定的 运动行为的方面。这项提案中的实验旨在 测试与“小脑模块”概念有关的假设 辨别脑干传入神经的组织原则 大鼠和小鼠的小脑后部皮质。这些实验是作为 以下是： 1.向单个脑干核团注射氚氨基酸 确定他们对小脑皮质的输入的组织。 2.注射小麦胚凝集素-辣根过氧化物酶(WGA-HRP)结合物 在同一小脑和不同小脑的不同内侧位置 小叶的位置，脑干细胞投射到这些 特定地区。 3.将示踪剂逆行注入两个预定的矢状面旁区域 确定单个神经元是否将Oxon侧支投射到更多 不只是一个区域。 4.小脑皮层注射WGA-HRP和氚氨基酸。 同一只动物的脑干来证实研究结果 上面列出了。 5.注射两种逆行示踪剂，一种在小脑皮质，另一种在大脑皮层 确定橄榄细胞是否投射侧支的小脑深部核 传到接受浦肯野细胞传入的深核细胞 由同一组橄榄细胞支配。 这些实验的仔细执行应该会提供有趣和 有关传入神经的解剖组织的令人兴奋的信息 输入到小脑后部皮质。它还应该提供一些 重要数据，我们可以根据这些数据来评估“小脑”的概念 “模块”作为小脑的基本功能单位和一些信息 关于这一职能的性质。希望这条信息能 使我们更清楚地了解小脑在调节运动中的作用 正常动物和有小脑或其他运动的动物的活动 疾病。

A number of afferent and efferent projections of the cerebellar cortex have been shown to terminate as a series of parasagittally oriented zones. The concept of a "cerebellar module" or "microzone" has been proposed as being the basic functional unit of the cerebellum which modulates a certain aspect of motor behavior. The experiments in this proposal are designed to test hypotheses relating to the concept of a "cerebellar module" and to discern the organizational principles of brainstem afferents to the posterior cerebellar cortex in the rat and mouse. These experiments are as follows: 1. Inject individual brainstem nuclei with tritiated amino acids to determine the organization of their inputs to the cerebellar cortex. 2. Inject wheat germ agglutinin-horseradish peroxidase (WGA-HRP) conjugate into different mediolateral positions in the same and different cerebellar lobules to map the location, of brainstem cells which project to these specific regions. 3. Inject to retrograde tracers into two predetermined parasagital zones to determine whether individual neurons project oxon collaterals to more than one zone. 4. Inject WGA-HRP in the cerebellar cortex and tritiated amino acids in the brainstem of the same animal to corroborate the results of the studies listed above. 5. Inject two retrograde tracers, one in cerebellar cortex and one in the deep cerebellar nuclei to determine if olivary cells project collaterals onto deep nuclear cells which receive afferents from the Purkinje cells innervated by the same group of olivary cells. The careful execution of the experiments should provide interesting and exciting information concerning the anotomical organization of afferent input to the posterior cerebellar cortex. It should also provide some important data upon which we can evaluate the concept of the "cerebellar module" as the basic functional unit of the cerebellum and some information concerning the nature of that function. Hopefully this information will lead to a clearer understanding of the cerebllum's role in modulating motor activity in the normal animal and in animals with cerebellar or other motor diseases.