PARASAGITTAL ORGANIZATION OF THE CEREBELLAR CORTEX

小脑皮质的一些传入和传出投射有 已经被证明终止为一系列的parasagitally定向区。 的 已经提出了“小脑模块”或“微区”的概念， 小脑的基本功能单位，调节某种 运动行为方面。 本提案中的实验旨在 测试与“小脑模块”概念相关的假设， 辨别脑干传入的组织原则， 大鼠和小鼠的小脑后皮质。 这些实验是 如下所示： 1. 向个别脑干细胞核注射氚化氨基酸， 决定了他们对小脑皮层的输入组织。 2. 注射麦胚凝集素-辣根过氧化物酶（WGA-HRP）结合物 在同一个和不同的小脑中的不同的内外侧位置 小叶来绘制脑干细胞的位置， 具体区域。 3. 将逆行示踪剂注入两个预先确定的矢状旁区 以确定单个神经元是否将oxon侧枝投射到更多的 不止一个区。 4. 小脑皮质内注射WGA-HRP， 同一只动物的脑干来证实研究结果 上面列出的。 5. 注射两个逆行示踪剂，一个在小脑皮质，一个在 小脑深部核以确定橄榄细胞是否投射侧支 传递到接受浦肯野细胞传入神经的深层核细胞上 由同一组橄榄细胞支配。 仔细执行的实验应该提供有趣的， 关于传入神经解剖学组织的令人兴奋的信息 输入到小脑后皮质。 它还应该提供一些 重要的数据，我们可以评估的概念，“小脑” 模块”作为小脑的基本功能单位和一些信息 关于这个功能的性质。 希望这些信息 从而更清楚地了解大脑在调节运动中的作用 活动在正常动物和动物与小脑或其他运动 疾病

A number of afferent and efferent projections of the cerebellar cortex have been shown to terminate as a series of parasagittally oriented zones. The concept of a "cerebellar module" or "microzone" has been proposed as being the basic functional unit of the cerebellum which modulates a certain aspect of motor behavior. The experiments in this proposal are designed to test hypotheses relating to the concept of a "cerebellar module" and to discern the organizational principles of brainstem afferents to the posterior cerebellar cortex in the rat and mouse. These experiments are as follows: 1. Inject individual brainstem nuclei with tritiated amino acids to determine the organization of their inputs to the cerebellar cortex. 2. Inject wheat germ agglutinin-horseradish peroxidase (WGA-HRP) conjugate into different mediolateral positions in the same and different cerebellar lobules to map the location, of brainstem cells which project to these specific regions. 3. Inject to retrograde tracers into two predetermined parasagital zones to determine whether individual neurons project oxon collaterals to more than one zone. 4. Inject WGA-HRP in the cerebellar cortex and tritiated amino acids in the brainstem of the same animal to corroborate the results of the studies listed above. 5. Inject two retrograde tracers, one in cerebellar cortex and one in the deep cerebellar nuclei to determine if olivary cells project collaterals onto deep nuclear cells which receive afferents from the Purkinje cells innervated by the same group of olivary cells. The careful execution of the experiments should provide interesting and exciting information concerning the anotomical organization of afferent input to the posterior cerebellar cortex. It should also provide some important data upon which we can evaluate the concept of the "cerebellar module" as the basic functional unit of the cerebellum and some information concerning the nature of that function. Hopefully this information will lead to a clearer understanding of the cerebllum's role in modulating motor activity in the normal animal and in animals with cerebellar or other motor diseases.